相关实验视频
Updated: Jun 26, 2025
07:56
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
21.7K
在课堂上,CRISPR/Cas9基因向加上纳米孔DNA测序与等离子体pBR322在课堂上
Röbbe Wünschiers1, Robert Maximilian Leidenfrost2, Hauke Holtorf3
1Biotechnology and Chemistry, University of Applied Sciences Mittweida, Mittweida, Germany.
Journal of microbiology & biology education
|May 10, 2024
概括
这项研究提出了一个简单的实验室协议,结合了CRISPR/Cas基因编辑和纳米孔DNA测序,用于教育目的. 学生可以学习如何阅读和编辑DNA,使用易于使用的工具和最小的成本.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 生物技术教育教育 生物技术教育
背景情况:
- 纳米孔DNA测序和CRISPR/Cas基因编辑是遗传学中的变革性技术.
- 将这些先进技术整合到学生的学习中,对于未来的科学素养至关重要.
研究的目的:
- 为学生开发一个简化的实践实验室协议,让他们学习DNA测序和基因编辑.
- 为了使现代分子遗传学可用,成本和计算要求最小.
主要方法:
- 为CRISPR/Cas9定位指导RNA的生物合成.
- 使用CRISPR/Cas9.9进行pBR322等离子体的特定线性化.
- 通过纳米孔测序识别DNA切割部位.
主要成果:
- 一个具有成本效益的协议 (约. 每组130美元) 不需要任何生物或生物安全实验室.
- 一个免费访问的网站,用于简化序列分析和可视化.
- 在高中,教师培训和大学成功实施.
结论:
- 联合的CRISPR/Cas和纳米孔测序实验有效地使学生参与现代分子遗传学.
- 该协议为学习DNA操纵和分析提供了一种实用,可访问和经济高效的教育工具.
相关概念视频
01:59CRISPR
50.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.7K
02:53CRISPR and crRNAs
17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K