在体外对牛Th2细胞的分化和调控
Anmol Kandel1, Lei Li1, Yan Wang2
1Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Cells
|May 10, 2024
概括
牛的Th2细胞不能一致地表达IL4蛋白,即使受到刺激. 这表明,与其他物种不同,IL4不是分化牛Th2细胞的决定性特征.
科学领域:
- 免疫学 免疫学 免疫学
- 兽医医学 兽医医学 兽医医学
- 细胞生物学 细胞生物学
背景情况:
- 牛的Th2细胞通常通过Interleukin-4 (IL4) mRNA表达来识别.
- 牛Th2细胞中IL4转录和蛋白质表达之间的相关性尚不清楚.
- 之前的研究表明,在牛中,IL4表达Th2细胞的频率较低.
研究的目的:
- 为了研究在不同组织中牛 CD4+ T 细胞中 IL4 的蛋白质表达.
- 为了确定牛CD4+T细胞在体外分化成Th2细胞的能力.
- 探索牛群IL4表达和Th2分化的调节机制.
主要方法:
- 流细胞计用于分析血液,脏和淋巴结中的IL4 + CD4 + T细胞.
- 在体外Th2差异化试验中,使用原始牛CD4+T细胞进行了用抗牛CD3和Th2差异化套件刺激的试验.
- 使用定量PCR,无偏向蛋白质组学和对T细胞受体刺激强度的操纵来评估差异化和IL4表达.
主要成果:
- 在被检查的牛组织中,在CD4+ T细胞中检测到有限的IL4蛋白.
- 实验室分化主要产生IFNγ生成细胞,其中不到10%的IL4共表达.
- 在分化后,GATA3的表达减少,外部病原体如*Ostertagia ostertagi*提取物抑制了关键信号通路.
结论:
- 牛的CD4+T细胞可以对Th2分化刺激做出反应,但IL4蛋白表达不是可靠的标记物.
- 牛中的IL4通路调节与在小鼠和人类中观察到的显著不同.
- 外部因素,如寄生虫感染,可以调节牛的Th2细胞分化.
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