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在大鼠模型中,介质干细胞外体增强后侧脊柱融合

Wing Moon Raymond Lam^1,2, Wen-Hai Zhuo^1,2, Long Yang^1,2

¹Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, 1E Kent Ridge Road, Singapore 119228, Singapore.

|May 10, 2024

概括

介酶体 stromal/干细胞 (MSC) 外体对脊髓融合手术有希望. 在大鼠模型中,单剂量在支架上增强了骨形成和机械稳定性,表明了转化潜力.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
整形外科手术整形外科手术

背景情况:

脊柱融合用于退行性腰部疾病经常使用重组人骨形态基因蛋白2 (rhBMP-2),它有不良影响.
介酶体 stromal/干细胞 (MSCs) 和它们的细胞外囊泡 (EVs),特别是外体,显示出骨和软骨修复的潜力.
在促进脊髓融合方面,MSC外体的有效性尚未确定.

研究的目的:

为了研究MSC外体的脊髓融合效率,通过聚烯酸酸 (PCL-TCP) 支架内通过原体海绵输送.
评估MSC外体的潜力,作为在脊髓融合中比rhBMP-2更安全的替代品.

主要方法:

使用的是老鼠后侧脊柱融合模型.
MSC外体被装载到可吸收的原体海绵上,并装入PCL-TCP支架中.
脚手架是在一个单一的管理中植入的.

主要成果:

用外体补充的支架单次植入显著增强了脊柱融合.
观察到骨形成的改善和横向过程之间的桥梁.
在融合得分,速率,骨结构,组织学,机械度和运动范围方面,有显著的改善.

结论:

MSC外体有效地促进骨形成,增强脊髓融合和机械稳定性.
这项研究提供了在老鼠脊柱融合模型中MSC外体效能的第一个证据.
在脊柱融合应用中,MSC外体具有翻译潜力.

关键词:

骨头骨头骨头骨头外基因组是外基因组的组成部分.细胞外囊泡中的细胞外囊泡.介质细胞干细胞介质细胞干细胞脊柱融合是指脊柱的融合.

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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...