相关实验视频

Updated: Jun 26, 2025

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

侵袭因子ODZ1通过表皮生长因子受体诱导的途径在原发性质母细胞瘤细胞中得到升级

Carlos Velasquez^1,2,3, Olga Gutierrez², Maria Carcelen²

¹Department of Neurosurgery, Hospital Universitario Marqués de Valdecilla, 39008 Santander, Spain.

|May 10, 2024

概括

皮表皮生长因子 (EGF) 调节ODZ1,一种驱动质母细胞瘤 (GBM) 侵袭的蛋白质. 这通过EGF受体 (EGFR) 和p38βMAPK通路发生,为GBM提供了新的治疗点.

科学领域:

神经瘤学神经瘤学
分子生物学分子生物学
细胞信号传递细胞信号传递

背景情况:

质母细胞瘤 (GBM) 侵袭是由细胞骨重塑驱动的,需要形态变化.
ODZ1蛋白通过RhoA-ROCK通路激活促进GBM细胞入侵.
IL-6和缺氧通过转录来调节ODZ1的表达.

研究的目的:

研究表皮生长因子 (EGF) 在调节ODZ1表达和GBM细胞入侵中的作用.
阐明了将EGF信号与ODZ1诱导联系起来的分子机制.

主要方法:

在20个GBM初级细胞系中使用流细胞计分析了EGF受体 (EGFR) 表达.
用EGF连接体处理EGFR表达和非表达的GBM细胞.
研究了Cetuximab,p38 MAPK通路抑制剂和MAPK11 (p38β MAPK) siRNA对ODZ1诱导的影响.

主要成果:

在20个GBM细胞系中,有19个表达EGFR.
刺激EGF增加了EGFR表达GBM细胞中的ODZ1mRNA和蛋白质水平.
阻断EGF-EGFR,抑制p38 MAPK,或击倒MAPK11减少了EGF诱导的ODZ1上调.

结论:

EGF激活了一条由EGFR介导的信号通路,涉及p38β MAPK,以对GBM细胞中的入侵因子ODZ1进行上调.
这一途径有助于形成形态变化,使GBM细胞能够侵入周围组织.
EGF-EGFR-p38β MAPK-ODZ1轴为GBM治疗提供了一个潜在的新治疗点.

关键词:

欧洲农业基金会 (EGFR) 是一个.这就是ODZ1的原因.质母细胞瘤是什么移民移民迁移迁移 p38 MAPKK 的一个.

更多相关视频

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells

Published on: January 10, 2018

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...