Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.7K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.7K
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Overview of Exosomes01:36

Overview of Exosomes

2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Beyond motor: a systematic review of multisensory integration deficits in Parkinson's disease.

Journal of neural transmission (Vienna, Austria : 1996)·2026
Same author

Direct C-H difluoromethylation of heteroaromatic compounds: emerging methods and developments through 2025.

RSC advances·2026
Same author

The association between serum trace elements and iron deficiency anemia in children and adolescents: a systematic review and meta-analysis.

Hematology (Amsterdam, Netherlands)·2026
Same author

Incidence of cranial and ophthalmic nerve palsy and associated risk factors in tuberculous meningitis: A systematic review and meta-regression analysis.

IBRO neuroscience reports·2026
Same author

Associations between perceived social support, self-efficacy, and health-promoting behaviors in hospitalized heart failure patients: a cross-sectional study.

BMC cardiovascular disorders·2026
Same author

Development of a Nano-Real-Time Polymerase Chain Reaction (RT-PCR) Kit for Detection and Genotyping of High-Risk Human Papillomavirus (HPV) Strains Using Dedicated TaqMan Probes.

Cureus·2026

相关实验视频

Updated: Jun 26, 2025

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
12:02

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

Published on: November 11, 2021

4.2K

介酶干细胞衍生的外体细胞通过诱导转化生长因子β2 (TGF-β2) 来降低牛皮中的超增生.

Zahraa Ibrahim Abed1, Mona Arianejad2, Zahra Azizi3

  • 1Department of Biology, Science and Research Branch, Islamic Azad University, P.O. Box 14515-775, Tehran, Iran.

Molecular biology reports
|May 10, 2024
PubMed
概括
此摘要是机器生成的。

介质细胞干细胞/细胞 (MSC) 外体显示出通过减少皮肤增生来治疗牛皮的潜力. 这些外生体调节转化生长因子β2 (TGF-β2) 信号传递,为免疫治疗提供了替代方案.

关键词:
外基因组是外基因组的组成部分.过度增生症 过度增生症介质细胞干细胞 介质细胞干细胞牛皮的病症是什么在TGF-β2中.

更多相关视频

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
09:48

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Published on: July 26, 2022

4.3K
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

2.0K

相关实验视频

Last Updated: Jun 26, 2025

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells
12:02

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

Published on: November 11, 2021

4.2K
Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
09:48

Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture

Published on: July 26, 2022

4.3K
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
07:03

Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Published on: September 23, 2022

2.0K

科学领域:

  • 生物医学科学 生物医学科学
  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 牛皮是一种慢性炎症性皮肤疾病,通过免疫疗法治疗.
  • 长期免疫疗法带来健康风险,并可能导致反应丧失.
  • 需要使用替代治疗方法,其中介质干细胞/干细胞 (MSC) 外体因其免疫调节性质而显得有前途.

研究的目的:

  • 为了研究MSC外体是否可以减少牛皮引起的增生.
  • 为了确定MSC外体是否诱导转化生长因子β2 (TGF-β2) 信号,以实现这种减少.

主要方法:

  • 通过超离心法从MSC中分离出外体.
  • 用扫描电子显微镜和布拉德福德测试来评估外体形态和度.
  • 使用实时PCR和ELISA分别量化TGF-β2表达和蛋白质水平.

主要成果:

  • 均的,杯状外体 (15-30 nm) 被成功分离出来.
  • MSC外体治疗显著增加了目标细胞中的TGF-β2基因表达.
  • 治疗过程中计算出的IC50值为181.750μg/ml.

结论:

  • MSC外体可以减少牛皮引起的增生症.
  • 通过TGF-β2信号传递对TGF-β2表达的升级是MSC外基因组发挥治疗作用的关键机制.