降低SOX9表达的调节,在与膜内骨相邻的发育性体中
Asahi Kitamura1, Masahito Yamamoto2,3, Hidetomo Hirouchi3
1Department of Removable Partial Prosthodontics, Tokyo Dental College, Chiyoda-ku, Tokyo, Japan.
PloS one
|May 10, 2024
概括
SOX9表达在关节 (TMJ) 凝结发育过程中受到负面调节,与纤维软骨凝结不同. 这项研究显示了SOX9的存在.
科学领域:
- 发育生物学是发展生物学.
- 结合组织生物学 结合组织生物学
- 整形外科 整形外科 整形外科
背景情况:
- 连接骨和肌肉至关重要的位被分为纤维软骨,纤维和周骨类型.
- 纤维和周骨内的发育机制,特别是在关节 (TMJ) 中,尚不清楚.
- SOX9是体发生的关键转录因子,但它在纤维和周骨内发育中的作用不太清楚.
研究的目的:
- 调查SOX9在关节 (TMJ) 结合的发展中的作用.
- 为了比较关节结合体中的SOX9表达模式与纤维结合体结合体中的SOX9表达模式.
- 为了阐明SOX9在纤维和周骨形成中的功能意义.
主要方法:
- 在关节内发育过程中对SOX9表达的分析.
- 使用Wnt1Cre;Sox9flox/+记者小鼠检查TMJ结合中的SOX9功能.
- 在野生类型和转基因小鼠中对体结构的组织学检查.
主要成果:
- SOX9的表达在关节内发育过程中受到负面调节,与纤维软骨内形成鲜明对比.
- 关节结节与膜内骨相邻,而不是软骨.
- Wnt1Cre;Sox9flox/+小鼠在关节中表现出肠道变形,这表明SOX9.9的作用.
- 观察到与关节骨膜骨相关的内中SOX9表达的减少.
结论:
- SOX9在纤维和骨周内的发展中发挥着独特的作用,与其在纤维软骨内中的作用不同.
- SOX9表达的调节取决于骨化模式.
- 这些发现突显了体的多样化发育途径和SOX9.9的上下文依赖功能.
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