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在果果发育过程中涉及糖和酸积累的转录景观和动态
Baiyun Li1,2, Lingcheng Zhu1, Nanxiang Yang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
研究人员确定了控制果糖和酸水平的关键转录因子. 这项研究揭示了监管网络对于果的开发和质量改善至关重要.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 生物化学 生化学
背景情况:
- 糖和酸对于肉质水果的味道和质量至关重要.
- 在水果发育过程中糖和酸积累的转录调节是不太了解的.
研究的目的:
- 为了研究全基因组的染色质可访问性,并确定调节果 (Malus domestica) 水果中糖和酸积累的转录因子.
- 构建一个转录性调节网络,用于选关键的调节基因.
主要方法:
- 结合ATAC-seq (使用测序测定转化酶可访问染色质的测试) 与RNA-seq (RNA测序).
- 整合了具有差异表达基因的差异可访问区域,以确定监管关系.
- 建立了一个转录监管网络.
主要成果:
- 确定了一个全球数据集的促进者可访问性和表达增加的基因.
- 构建了糖和酸积累的转录性调节网络.
- 证实了5种特定于水果的DNA与一根手指基因结合,通过影响与代谢相关的基因来调节糖或酸度.
结论:
- 开发的转录性调节网络是识别控制果糖和酸积累的候选基因的宝贵平台.
- 这些发现提高了对果开发中的监管动态的理解.
- 这项研究为改善果质量奠定了基础.
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