使用多次时间分辨率的光微球免疫染色学试验带快速地在现场定量测定谷物中的真菌毒素
JunFa Liang1, Xuewei Li2, Bin Huang3
1Guangzhou Institute of Food Inspection, Guangzhou, 511400, PR China.
Biosensors & bioelectronics
|May 10, 2024
概括
这项研究引入了一种新的时间分辨率光微球测试条,用于检测谷物中的多种真菌毒素. 它提供敏感,快速和稳定的在现场检测阿弗拉托辛B1,泽拉伦和脱氧尼瓦伦.
科学领域:
- 分析化学 分析化学
- 生物传感技术的技术
- 食品安全 食品安全
背景情况:
- 侧流免疫检测 (LFIA) 对于敏感的检测至关重要,但传统的探测器有局限性.
- 光微球 (FMs) 面临着诸如短光寿命和背景干扰等挑战.
- 在现有的基于FM的LFIA中,多组件检测往往很困难.
研究的目的:
- 开发一种基于多重时间分辨率光微球的新型免疫染色体测试条 (TRFM-ICTS).
- 为了快速地在现场定量检测谷物中的三个关键菌毒素.
- 在LFIA中克服传统光探针的局限性.
主要方法:
- 合成了碳酸盐修饰的Eu(III) 酸盐合聚烯纳米珠,并用作标签探针.
- 构建了一个基于多个时间分辨率的光微球免疫染色学试验条 (TRFM-ICTS).
- TRFM-ICTS用于在谷物中同时检测阿弗拉托辛B1 (AFB1),泽拉伦 (ZEN) 和脱氧尼瓦伦 (DON).
主要成果:
- 达到的检测极限 (LOD) 为AFB1的0.03 ng/g,ZEN的0.11 ng/g,DON的0.81 ng/g.
- 已经证明了广泛的检测范围:AFB1 (0.05-8.1 ng/g),ZEN (0.125-25 ng/g) 和DON (1.0-234 ng/g).
- 具有出色的选择性和显著的稳定性,在4°C保存一年以上后仍然保持检测能力.
结论:
- 开发的TRFM-ICTS提供了一种灵敏,准确和简单的在现场检测真菌毒素的方法.
- 测试带是便携式的,可以在20分钟内使用便携式阅读器进行检测.
- 为需要快速菌毒素分析的实际食品安全应用提供了一个有前途的解决方案.
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