NRP1是哺乳动物orthoreovirus的受体,由不同的体子单元参与
Pengcheng Shang1, Rita Dos Santos Natividade2, Gwen M Taylor1
1Department of Pediatrics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Institute of Infection, Inflammation, and Immunity, UPMC Children's Hospital of Pittsburgh, Pittsburgh, PA, USA.
Cell host & microbe
|May 10, 2024
概括
哺乳动物的ortoreovirus使用神经1 (NRP1) 作为一个入口受体. 这种相互作用是重复病毒传播和小鼠中枢神经系统 (CNS) 感染的关键.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 神经科学是一个神经科学.
背景情况:
- 哺乳动物的ortoreovirus (reovirus) 是一种非外病毒,会引起肠道感染,并扩散到包括中枢神经系统 (CNS) 在内的次要部位.
- 重复病毒系统传播和神经元向的精确机制尚未完全理解.
- 病毒进入通常涉及多个宿主细胞参与因素.
研究的目的:
- 为了确定reovirus用于进入和全身传播的特定宿主细胞受体.
- 为了阐明基底的分子机制reovirus-neuropilin 1 (NRP1) 相互作用.
- 确定NRP1在reovirus发病过程中的作用,特别是神经病毒发病.
主要方法:
- 鉴定小鼠神经蛋白1 (mNRP1) 作为一个reovirus结合蛋白.
- 病毒与受体结合亲和力和相互作用接口的表征.
- 对reovirus囊蛋白 (σ3和λ2) 的基因操纵,以评估结合和传染性.
- 使用囊突变体的体内研究来评估小鼠中的reovirus传播和神经病毒性.
主要成果:
- 鼠神经素1 (mNRP1) 被确定为对reovirus的高亲和感受体.
- 雷奥病毒-NRP1相互作用涉及病毒囊蛋白 (σ3和λ2) 和NRP1.1的细胞外域之间的多价值结合.
- 在小鼠模型中,无法结合NRP1的体突变体显示出系统传播和神经病毒性减少.
- NRP1在促进reovirus进入和促进其病变发生方面发挥着重要作用.
结论:
- 神经皮林1 (NRP1) 作为哺乳动物orthoreovirus的关键入口受体.
- 重复病毒囊蛋白和NRP1之间的多价值相互作用对于病毒传播和神经毒性至关重要.
- 了解NRP1的作用可以了解病毒病原和潜在的治疗策略.
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