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特里顿X-100可以抵消Enterococcus faecalis的抗生素耐药性:一个体外试验研究

Xinling He¹, Shujie Xv¹, Runze Liu¹

¹The State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.

Journal of dentistry

|May 10, 2024

概括

特里顿X-100 (TX-100) 通过增加细胞透性和减少能量生产,增强抗生素对抗耐药性Enterococcus faecalis的有效性. 这种辅助策略打击抗生素耐药性和病原性,为新的牙科消毒剂提供了潜力.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

像Enterococcus faecalis这样的细菌的抗生素耐药性是全球主要的健康问题.
辅助剂可能会恢复抗生素对抗耐药菌株的有效性.
了解耐药机制对于开发有效的治疗方法至关重要.

研究的目的:

调查Triton X-100 (TX-100) 对抗生素活性对抗耐药Enterococcus faecalis的辅助作用.
阐明TX-100增强抗生素作用并降低病原性的分子机制.

主要方法:

在体外测试 gentamicin 硫酸盐和 ciprofloxacin 化物与或没有 TX-100 对浮游生物和生物膜 E. faecalis.
转录组和代谢组分析以确定TX-100的分子标.
评估膜性质,能量代谢 (ATP) 和病毒性基因表达.

主要成果:

低度的TX-100显著增强了对抗耐药性E. faecalis.抗 gentamicin和ciprofloxacin的抗菌活性.
TX-100增加了细菌膜的透性,并消散了膜潜力.
通过降低ABC载体,转移酶系统 (PTS) 和ATP生产的调节,TX-100减弱了抗生素耐药性和致病性.

结论:

TX-100作为一种有效的抗生素辅助剂,提高易感性,降低E. faecalis的耐药性和病原性.
这些发现支持开发新型根管消毒剂,包括TX-100来对抗抗生素耐药性.

关键词:

抗生素抗生素是一种抗生素.菌 (Enterococcus faecalis) 是一种菌.代谢调节代谢调节代谢调节电阻电阻电阻电阻电阻电阻特里顿X-100可以使用.

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