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squash 多糖的结构特征及其对 STZ 诱导的糖尿病模型在 MIN6 细胞的影响

Yu Zhang¹, Bingjie Yang¹, Wei Sun²

¹College of Food Science and Nutritional Engineering, China Agricultural University, China; China National Engineering Research Center for Fruit and Vegetable Processing, China; Key Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture, Beijing 100083, China.

International journal of biological macromolecules

|May 10, 2024

概括

一种新的南瓜多糖 (PWESP-3) 在糖尿病模型中增强胰岛素分泌和细胞活力. 这种天然化合物可以防止氧化应激和DNA损伤,为糖尿病管理提供作为食品补充剂的潜力.

科学领域:

碳水化合物化学碳水化合物化学
生物化学生物化学
药理学药理学是指药理学的学科.

背景情况:

1型糖尿病是一种慢性疾病,其特征是胰岛素缺乏.
氧化应激和DNA损伤在糖尿病的发病过程中起着至关重要的作用.
天然化合物正在被探索为糖尿病管理的替代治疗剂.

研究的目的:

从南瓜中分离和描述一种新型的多糖体 (PWESP-3).
为了研究PWESP-3对MIN6细胞中链毒素 (STZ) 诱导的1型糖尿病的保护作用.
评估PWESP-3作为糖尿病功能补充剂的潜力.

主要方法:

从南瓜中分离和净化PWESP-3.
使用NMR光谱学阐明PWESP-3的结构.
在体外评估PWESP-3对暴露于STZ的MIN6细胞的影响,包括活力,胰岛素分泌,氧化应激标志物 (ROS,NO),ATP含量,Ca2+水平,线粒体膜潜力,Caspase-3活性和DNA损伤.

主要成果:

一种富含银河糖和氨酸的水溶性酸性多糖体 (140.519 kDa) PWESP-3 成功被分离和表征.
PWESP-3显著增加了MIN6细胞活力和胰岛素分泌.
通过调节ATP,Ca2+,线粒体膜潜力和Caspase-3活性,PWESP-3减少了MIN6细胞中STZ诱导的氧化应激,亡和DNA损伤.

结论:

PWESP-3在MIN6细胞中显示出对STZ诱导的损伤具有显著的保护作用.
PWESP-3 改善胰岛素分泌功能,减轻氧化应激和DNA损伤,表明其治疗潜力.
PWESP-3可以被视为一种有希望的天然替代食品补充剂,用于管理1型糖尿病.

关键词:

低血糖低血糖是指低血糖的情况.在 MIN6 单元中,squash squash 的多糖类是什么?结构结构是一个结构.第一种类型糖尿病.

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