不常见的素的视网膜同位素参与了哺乳动物精子的热毒作用
Alexander Brandis1, Debarun Roy2,3, Ishita Das4
1Department of Life Sciences Core Facilities, The Weizmann Institute of Science, 7610001, Rehovot, Israel.
Scientific reports
|May 10, 2024
概括
哺乳动物的精子使用opsins作为热传感器进行热毒性,这个过程取决于色素体视网膜. 在精子中发现了一种独特的tri-cis视网膜同位素,这表明它在温度感应中的作用.
科学领域:
- 精子学 精子学
- 分子生物学分子生物学
- 感官生物学 感官生物学
背景情况:
- 通常被称为视觉光传感器的opsins在哺乳动物精子中发现.
- 这些opsins作为高度敏感的热传感器起作用,调解精子热毒作用.
- 素作为精子中的热传感器的机制,与它们的光传感器作用不同,仍然不清楚.
研究的目的:
- 为了研究以素为基础的热感应在精子中的分子基础.
- 为了确定视网膜,对光素光敏性的染色体,是否在精子热毒性中发挥作用.
- 为了确定精子中存在的特定形式的视网膜.
主要方法:
- 功能性测试以评估精子热毒性.
- 质谱学 (LC-MS) 检测和识别精子提取物中的视网膜.
- 超高性能收色谱用于表征视网膜异构体.
- 在小鼠中进行的维生素A (视网膜前体) 饥饿和补充研究.
主要成果:
- 在维生素A饥饿时,精子热致性丧失,在补充时恢复.
- 在小鼠和人类精子中使用LC-MS检测了视网膜.
- 在精子中发现了一个独特的tri-cis视网膜同位素,与视觉的11-cis同位素不同.
结论:
- 视网膜对于哺乳动物的精子热致作用至关重要.
- 鉴定到的三-cis视网膜同位素,是精子细胞独有的,与它们的热感知能力有关.
- 精子中的奥普辛利用视网膜来感知温度,扩大它们已知的功能作用.
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