一个Trypanosoma sp.的形态和分子特征. 来自中国的三胺 (Triatoma rubrofasciata) 的
Yunliang Shi1,2, DeHua Lai3, Dengyu Liu1
1Parasitology Department, School of Basic Medical Sciences, Guangxi Medical University, Nanning, 530021, People's Republic of China.
Parasites & vectors
|May 10, 2024
概括
这项研究证实了中国Trypanosoma conorhini的存在.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 载体传播疾病 载体传播疾病
背景情况:
- 三原子体 (接吻虫) 是三生体的载体.
- 在中国,Trypanosoma conorhini和Triatoma rubrofasciata的传播周期尚不清楚.
研究的目的:
- 在中国的Triatoma rubrofasciata中识别和描述试体物种.
- 为了研究Trypanosoma conorhini的传播动态.
主要方法:
- 对Triatoma rubrofasciata寄生虫的形态分析.
- DNA提取,PCR放大 (18S rRNA,HSP70,gGAPDH),并进行测序.
- 生殖基因分析和BLAST识别.
- 用老鼠/小鼠和Triatoma rubrofasciata进行实验室感染实验.
- 在Triatoma rubrofasciata.中试体感染的流行率研究.
主要成果:
- 试虫种类 (Trypanosoma sp.) 是一种虫. 在中国广西的Triatoma rubrofasciata中发现的,在形态上与表观虫相一致.
- 分子分析证实了这种寄生虫是Trypanosoma conorhini,与T. cruzi和T. rangeli.不同.
- 罗布罗法萨蒂亚菌 (Triatoma rubrofasciata) 将Trypanosoma conorhini传播给老鼠和小鼠,并通过食用它们而受到感染.
- 在现场采集的Triatoma rubrofasciata中发现Trypanosoma conorhini感染的高患病率 (36.3%).
- 没有证据表明Triatoma rubrofasciata中存在过卵巢传播.
结论:
- 在中国的Triatoma rubrofasciata中首次报告Trypanosoma conorhini,由形态和分子数据支持.
- 传染可能发生在Triatoma rubrofasciata和动物之间,而不是通过跨卵巢途径.
- 载体的高流行率和已记录的人类咬伤引起了人们的担忧,需要对人类病原性进行进一步调查.
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