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Updated: Jun 26, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

LINC01021 减弱表达并影响p53-调节基因子集的替代分离

Markus Kaller¹, Ignasi Forné², Axel Imhof²

¹Experimental and Molecular Pathology, Institute of Pathology, Faculty of Medicine, Ludwig-Maximilians-Universität München, Thalkirchner Strasse 36, D-80337 Munich, Germany.

|May 11, 2024

概括

长非编码RNA LINC01021 调节结直肠癌中p53诱导的基因表达. 它的损失通过改变基因表达和拼接来增强化疗敏感性.

科学领域:

分子生物学分子生物学
癌症研究癌症研究
基因组学就是基因组学.

背景情况:

在p53-inducible长非编码RNA LINC01021影响敏感性对破坏DNA的化疗在结肠直肠癌 (CRC) 细胞系.
了解LINC01021在p53转录程序中的作用对于CRC治疗策略至关重要.

研究的目的:

综合分析LINC01021如何影响CRC中的p53诱导的转录程序.
为了研究废除LINC01021的p53-诱导性对基因表达和拼接的影响.

主要方法:

使用CRISPR/Cas9来删除SW480CRC细胞中LINC01021促进体中的p53结合部位.
在异位p53激活后进行RNA测序 (RNA-Seq).
通过RNA亲和力净化,然后通过质谱测量确定了与LINC01021相关的蛋白质.

主要成果:

在p53激活后,LINC01021 p53诱导性的丧失使调节的mRNA数量增加了约1.8倍.
当LINC01021的诱导性丧失时,直接的p53点基因 (例如,NOXA,FAS) 显示出更强的诱导.
在LINC01021 p53-inducibility丧失后,观察到特定mRNA (例如ARHGAP12,HSF2,LYN) 的替代拼接.
质谱学确定了参与mRNA前剪接的RNA结合蛋白作为LINC01021相互作用伙伴.

结论:

LINC01021似乎限制了p53介导的转录变化的程度和强度.
这种调节是通过对表达的上下文依赖的控制和p53调节基因子集的剪接来实现的.

关键词:

在这里,我们可以看到 LINC0102121 的链接.紫色的紫色的在ncRNA中,我们可以在 p53 里,p53 是一个 p53 的类型.瘤抑制瘤抑制

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