开放和封闭卵细胞玻璃化系统对胚胎发育的影响:系统性审查和网络元分析
Konstantinos Pantos1, Evangelos Maziotis1,2, Anna Trypidi2
1Centre for Human Reproduction, Genesis Athens Clinic, Papanikoli, 15232 Athens, Greece.
Journal of clinical medicine
|May 11, 2024
概括
与开放系统相比,封闭的玻璃化系统在卵细胞冷保存方面显示出更好的结果. 封闭系统保持卵细胞的能力,导致更高的胚胎细胞形成率,这对于成功的生育治疗至关重要.
科学领域:
- 生殖生物学 生殖生物学
- 低温生物学 低温生物学
- 在体外受精中进行体外受精.
背景情况:
- 卵细胞冷保存利用开放式和封闭式玻璃化系统.
- 关于它们对卵细胞能力和临床结果的影响的比较数据有限.
研究的目的:
- 为了比较开放式与封闭式玻璃化系统对实验室和临床结果的影响.
- 分析冷却和升温率在卵细胞玻璃化中的影响.
主要方法:
- 在PubMed/MEDLINE和Cochrane中央图书馆进行系统的文献搜索 (至2023年1月).
- 网络元分析,将玻璃化系统与新鲜卵子进行比较.
- 包括23项研究.
主要成果:
- 这两种玻璃化系统的受精率都低于新鲜卵子.
- 开放式和封闭式系统之间生存率没有显著差异.
- 与新鲜对照组相比,开放系统的裂变和囊胞率较低;封闭系统没有.
结论:
- 封闭的玻璃化系统似乎对卵细胞能力的影响较小.
- 与开放系统相比,更高的胚胎细胞形成率证明了这一点.
- 封闭系统的标准化可以优化卵细胞玻璃化实践.
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