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两种光探针用于识别乙胆酶:设计,合成和比较评估

Xia Lin^1,2,3, Qingyuan Yi¹, Binyang Qing⁴

¹Guangxi Key Laboratory of Special Biomedicine, Medical College, Guangxi University, Nanning 530004, China.

Molecules (Basel, Switzerland)

|May 11, 2024

概括

开发了新的探针来检测乙胆酶 (AChE),一种与肺癌细胞亡相关的酶. 一个探测器BF2-cur-Ben显示了细胞内和体内ACHE检测的卓越性能.

科学领域:

生物化学生物化学
化学生物学化学生物学
分子探头分子探头

背景情况:

乙胆酶 (AChE) 是神经递质中的关键酶,已与包括癌症在内的各种疾病有关.
开发用于AChE检测的选择性和敏感探针对于理解其在生物过程和疾病状态中的作用至关重要.
现有的ACHE检测方法可能缺乏选择性,敏感性或适用于复杂的生物环境.

研究的目的:

设计和合成用于选择性识别乙胆酶 (AChE) 的新型"开关"光探针.
评估这些探针的性能,包括它们的选择性,pH独立性,灵敏性和酶亲和力.
研究探针-AChE相互作用的机制,并评估它们在细胞内和体内应用中的潜力.

主要方法:

合成了两种新的基于BF2的光探针,BF2-cur-Ben和BF2-cur-But.
探针性能的表征包括选择性,pH依赖性,线性范围,检测极限 (LOD) 和酶亲和力 (Km).
使用高性能液体染色学 (HPLC),质谱学 (MS),分子模拟和斑马鱼实验的机械研究.

主要成果:

这两种探测器都在特定的线性范围内展示了选择性和pH独立的AChE识别.
与BF2-cur-But.Ben相比,BF2-cur-Ben表现出较低的LOD (0.031 U/mL),更高的酶亲和力 (Km = 16 ± 1.6 μM) 和更高的抑制剂敏感性.
分子模拟显示,BF2-cur-Ben的增强结有助于其优越的酶亲和力和细胞膜透 (较高的log p).
探针成功检测到细胞内AChE,并监测其在肺癌细胞亡期间的过度表达.
在斑马鱼实验中证实了BF2-cur-Ben的体内适用性.

结论:

开发的基于BF2的探针为ACHE检测提供了一种敏感和选择性的方法.
BF2-cur-Ben在灵敏度,亲和力和细胞透性方面表现出显著的优势,使其成为生物研究的一个有前途的工具.
这些探针有可能在细胞和体内模型中监测ACHE水平,有助于研究癌症等ACHE相关疾病.

关键词:

乙烯基胆化酶的使用灭症 (apoptosis) 是一种死亡的过程.比较评价比较的评价比较的评价肺癌细胞癌的肺癌.探测器探测器探测器探测器视觉化的监控监控是可视化的.

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