使用TOF MS探测器进行全面的二维气色谱 - - 一个有效的工具来追踪葡萄品种的签名
Daniela Fonseca1, Nuno Martins2, Raquel Garcia2,3
1Mediterranean Institute for Agriculture, Environment and Development & Institute of Research and Advanced Training, University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal.
这项研究开发了一种分析葡萄挥发性化合物的新方法,在五种红葡萄品种中确定了52种化合物. 结果显示,不同的品种特征因葡萄种类和收获年份而异.
科学领域:
- 食品化学 食品化学
- 分析化学 分析化学
- 葡萄种植 葡萄种植 葡萄种植
背景情况:
- 葡萄品种的挥发性化合物是葡萄酒香味和风味的关键.
- 了解这些化合物有助于葡萄质量评估和葡萄酒生产.
研究的目的:
- 开发和优化分析红葡萄品种中自由挥发性化合物的方法.
- 来自不同收获的Trincadeira,Cabernet Sauvignon,Syrah,Castelão和Tinta Barroca葡萄的挥发性概况的特征.
主要方法:
- 头部空间固相微提取与气相色谱-气相色谱-飞行时间质谱相结合 (HS-SPME-GC × GC-TOFMS).
- 优化样品制备 (葡萄数量,NaCl,H2O) 和提取条件 (时间,温度,纤维类型) 的优化.
主要成果:
- 确定了52种自由挥发性化合物,17种单二烯,28种六二烯和7种C13-norisoprenoids.
- 与2022年相比,在2021年的收获中发现了更多的挥发性化合物.
- 线性差异分析 (LDA) 揭示了葡萄品种和收获年份之间挥发性概况的显著差异.
结论:
- 优化的HS-SPME-GC × GC-TOFMS方法有效地描述葡萄中的品种挥发性化合物.
- 葡萄品种和收获年份显著影响了挥发性概况,影响了品种特征.
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