相关实验视频

Updated: Jun 26, 2025

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

爱斯坦-巴尔病毒DNA在小鼠模型中通过类似收纳器9的收纳器加剧了关节炎

Nour Sherri¹, Rayan Assaf¹, Elio R Bitar¹

¹Department of Experimental Pathology, Immunology, and Microbiology, American University of Beirut, Beirut 1107, Lebanon.

International journal of molecular sciences

|May 11, 2024

概括

在小鼠模型中,托尔类受体-9 (TLR9) 抑制减少了埃普斯坦-巴尔病毒 (EBV) DNA 加剧的关节炎. 这表明TLR9是EBV感染个体的类风湿性关节炎的潜在治疗标.

科学领域:

免疫学免疫学免疫学
类风湿病学类风湿病学
病毒学病毒学

背景情况:

爱斯坦-巴尔病毒 (EBV) DNA的重新激活与通过托尔类受体-9 (TLR9) 增加IL-17A的产生有关.
埃博病毒感染与恶化自身免疫性疾病 (如类风湿性关节炎) 有关.

研究的目的:

在EBVDNA恶化原蛋白诱导性关节炎 (CIA) 鼠标模型中研究TLR9抑制的治疗潜力.
为了确定阻断TLR9是否可以减轻由EBV DNA驱动的关节炎的严重程度.

主要方法:

建立了一个原诱导关节炎 (CIA) 鼠标模型.
小鼠接受了原蛋白,EBV DNA + 原蛋白,EBV DNA + 原蛋白 + TLR9 抑制剂或单独使用TLR9 抑制剂的治疗.
临床,组织学和免疫学参数在70天后进行评估.

主要成果:

在患有EBV DNA诱导的关节炎的小鼠中,TLR9抑制显著降低了脚厚度,临床分数和关节损伤.
在TLR9抑制剂组中,握力显著改善.
在TLR9抑制后,在关节组织中观察到IL-17A,IFN-γ和FOXP3的联合表达减少.

结论:

在CIA模型中,抑制TLR9有效地限制了EBV DNA引起的关节炎恶化.
在患有EBV感染的个体中,TLR9代表了管理类风湿性关节炎的有希望的治疗标.

关键词:

在 EBV DNA DNA 中.它们的IL-17A是IL-17A.收费类受体9 收费类受体9 这是自身免疫力.类风湿性关节炎类风湿性关节炎

更多相关视频

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...