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克里斯普尔屏幕识别了RNA结合蛋白Eef1a1作为肌体发生的关键调节剂

Weiwei Liu^1,2, Wei Wang^2,3,4, Zishuai Wang^2,4

¹Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science & Technology, Guangxi University, Nanning 530004, China.

International journal of molecular sciences

|May 11, 2024

概括

研究人员确定Eef1a1是一种新型RNA结合蛋白 (RBP),调节骨肌肉发育. Eef1a1促进肌细胞增殖并抑制分化,与miR-133a-3p相互作用以控制肌细胞生成.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
发展生物学发展生物学

背景情况:

骨肌肉肌体发生涉及复杂的基因调节.
转录因子和非编码RNA的作用是已知的,但RNA结合蛋白 (RBPs) 在肌体发生过程中尚未得到研究.
调查后转录调节器对于理解肌体发生学至关重要.

研究的目的:

为了确定新型的功能性RBP调节肌肉发生.
为了研究Eef1a1在肌肉形成中的作用.
阐明Eef1a1涉及微RNA的调节机制.

主要方法:

在CRISPR高通量RBP全局淘汰库选中.
在细胞模型中使用CRISPR淘汰 (CRISPRko) 和CRISPR干扰 (CRISPRi).
siRNA 抑制,过度表达,RNA 免疫沉降 (RIP),miRNA 下拉,以及双露西法酶记者测定.

主要成果:

Eef1a1被确定为肌肉发生的一个新型调节因子.
Eef1a1促进了C2C12核细胞的增殖,并抑制了C2C12核细胞的分化.
证实miR-133a-3p是针对Eef1a1的,它可以拯救Eef1a1的影响.

结论:

Eef1a1是一种新型的RBP,它促进肌细胞增殖并抑制分化.
在肌肉生成的调节中,Eef1a1充当miR-133a-3p的对手.
这项研究揭示了骨肌肉发育中的新调节途径.

关键词:

这是一个CRISPR屏幕.这就是Eef1a1a1a1a.这是一种RNA结合蛋白.肌肉发育 (myogenesis) 是一个过程.

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