索马托斯坦素受体基因功能在双贝和的生长调节中的作用
Xiangchao Zhang1, Yuli Niu1, Can Gao1
1MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
International journal of molecular sciences
|May 11, 2024
概括
研究人员确定了体静止素受体-1 (SSTR-1) 作为双动物的生长抑制剂. 抑制SSTR-1显著增加了贝和的生长,为水产养殖繁殖提供了潜力.
科学领域:
- 海洋生物学 海洋生物学
- 水产养殖遗传学 水产养殖遗传学
- 分子内分泌学分子内分泌学
背景情况:
- 双鱼在海洋水产养殖中至关重要,但了解它们的生长机制是选择性繁殖的关键.
- 索马托斯坦素受体 (SSTR) 是已知的脊椎动物生长调节器,但它们在无脊椎动物 (包括双动物) 中的作用基本上是未知的.
- 在双动物中识别与生长相关的基因可以提高水产养殖的生产力.
研究的目的:
- 为了识别和描述Yesso海 (Patinopecten yessoensis) 中的体静止素受体 (SSTR) 基因.
- 研究SSTR-1在双生长调节中的功能.
- 探索SSTR-1作为改善双养殖的目标的潜力.
主要方法:
- 在Patinopecten yessoensis中发现了七个SSTR基因 (PySSTRs).
- 基因表达分析比较了快速生长与缓慢生长的海中的PySSTR-1水平.
- 在矮海 (Mulinia lateralis) 中使用RNA干扰 (RNAi) 通过食dRNA表达大肠杆菌来抑制MlSSTR-1.
- 在MlSSTR-1抑制后进行了转录组分析.
主要成果:
- 在快速生长的海中,PySSTR-1表达率较低.
- 在中抑制MlSSTR-1导致贝尺寸和组织重量显著增加 (高达92%).
- 在MlSSTR-1抑制后,高调基因在脂肪消化/吸收和胰岛素信号通路中得到了丰富.
结论:
- 在双动物中,SSTR-1 作为一种保守增长抑制剂,类似于它在脊椎动物中的作用.
- 通过dSRNA表达细菌的RNAi是一种有效的方法,用于对双动物的功能基因分析.
- SSTR-1 是一个有希望的候选基因,可以编辑以促进双鱼水产养殖的生长.
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