希斯乳酸化参与小鼠卵细胞成熟和胚胎发育
Diqi Yang1, Haoyi Zheng2, Wenjie Lu1
1School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
International journal of molecular sciences
|May 11, 2024
概括
乳化是一种新的表观遗传修饰,在小鼠卵细胞成熟和早期胚胎发育中发挥着动态作用. 补充乳酸改善了发育速度和改变了基因转录,为提高生殖质量提供了见解.
科学领域:
- 表观遗传学和发育生物学
- 细胞和分子生物学 细胞和分子生物学
背景情况:
- 翻译后的修改对于卵细胞成熟和胚胎发育至关重要.
- 乳化是一种最近发现的表观遗传修饰,在细胞过程中起着调节作用.
- 乳化在卵细胞成熟和早期胚胎发生过程中的存在和功能以前是未知的.
研究的目的:
- 调查小鼠卵细胞成熟和胚胎早期发育期间的氨酸乳化 (Kla) 的发生和动态.
- 通过使用外源乳酸盐,探索调节组素乳化水平对这些过程的影响.
- 了解乳化对与代谢途径相关的基因转录的影响.
主要方法:
- 免疫光染色用于检测小鼠卵细胞和胚胎中的乳酸蛋白和特定的基因素乳酸化标记 (H3K9la,H3K14la,H4K8la,H4K12la).
- 对卵细胞和胚胎进行了转录基因分析,这些卵细胞和胚胎与外源乳酸或无外源乳酸培养.
- 评估了卵细胞成熟率和早期胚胎发育.
主要成果:
- 乳酸蛋白在小鼠中表现出组织和细胞特异分布.
- 在卵细胞成熟的生殖囊泡 (GV) 阶段,基因素乳化水平最高,随后下降.
- 用10毫米的乳酸补充剂增加了卵细胞成熟率,增加了GV和第II (MII) 基相卵细胞中的基因素乳化,并增强了早期胚胎发育.
- 乳糖化调节改变了参与氧化酸化和糖解的基因的转录.
结论:
- 在小鼠卵细胞成熟和早期胚胎发生过程中,乳化是动态调节的.
- 外源乳酸补充剂可以通过增加乳化水平来增强卵细胞成熟和早期胚胎发育.
- 这些发现为乳糖在生殖过程中的作用提供了新的见解,并提出了改善卵细胞和胚胎质量的潜在策略.
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