相关实验视频

Updated: Apr 8, 2026

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

Published on: June 5, 2021

唾液作为在SARS-CoV-2检测中的生物液体

Emily Thalia Teixeira Silva¹, Fabiana Barcelos Furtado¹, Rosana Antunes da Silveira¹

¹Laboratory of Applied Biotechnology, Medical School, São Paulo State University (Unesp), Botucatu 18618-689, Brazil.

Diagnostics (Basel, Switzerland)

|May 11, 2024

概括

唾液检测为诊断SARS-CoV-2提供了一种可持续且具有成本效益的替代方案,显示了与鼻口腔拭子的定性诊断等价性. 这种方法推用于COVID-19检测.

科学领域:

病毒学病毒学
传染性疾病传染性疾病
诊断方法诊断方法

背景情况:

鼻切片的聚合酶链反应 (PCR) 是COVID-19大流行期间SARS-CoV-2诊断的黄金标准.
唾液采集已成为采样的一个有希望的替代方案.
这项研究旨在比较唾液和鼻抽样 (NPS) 在SARS-CoV-2检测中的有效性.

研究的目的:

为了比较使用唾液与NPS样本检测SARS-CoV-2的定性和定量检测.
为了确定两种样本采集方法的检测极限.
与NPS相比,评估唾液收集的财务成本和可持续性.

主要方法:

评估了99个配对的唾液和NPS样本以进行定性SARS-CoV-2检测.
对病毒颗粒进行了定量比较,并确定了检测极限.
比较周期值 (C_T) 值,疫苗接种情况和财务成本.

主要成果:

在唾液和抽样采集之间观察到质量诊断等价性 (96.96%).
两种方法的检测极限是相同的,尽管定量一致性很低.
在C_T值和疫苗接种状态之间没有发现显著的关联.
唾液收集显示了较低的财务成本和减少塑料材料的使用,表明更大的可持续性.

结论:

唾液收集是COVID-19诊断的可行和可持续的替代方案.
这些发现支持采用基于唾液的测试来检测SARS-CoV-2.

关键词:

在 COVID-19 疫情中,RT-qPCRR 是一个人.循环门值是指一个周期的门值.诊断诊断诊断诊断诊断诊断

更多相关视频

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

相关概念视频

Salivary Glands and Saliva

Salivary Glands and Saliva

The salivary glands, of which there are three pairs known as the parotid, submandibular, and sublingual glands, play a crucial role in maintaining oral health and initiating the digestive process. Positioned near the ears, beneath the masseter muscle, the parotid glands secrete saliva into the oral cavity through the parotid duct of Stensen. Meanwhile, the submandibular glands, located on the floor of the mouth, secrete saliva through channels named submandibular ducts. The sublingual glands,...