效应器AvrPik-D针对大米鲁比斯科小子单位OsRBCS4抑制免疫力
Linlin Song1, Tao Yang1, Xinxiao Wang1
1State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Plants (Basel, Switzerland)
|May 11, 2024
概括
米爆真菌Magnaporthe oryzae使用效应器AvrPik-D来准OsRBCS4蛋白质. 这种相互作用抑制了大米的免疫力,允许真菌感染植物并降低作物产量.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 生物化学 生物化学
背景情况:
- 由Magnaporthe oryzae引起的水爆发严重影响全球水产量.
- 效应物是抑制宿主免疫力的关键毒性因素.
- 通过M. oryzae效应器操纵宿主目标的精确机制在很大程度上是未知的.
研究的目的:
- 为了阐明M. oryzae效应剂AvrPik-D及其米标之间的相互作用.
- 为了了解AvrPik-D如何促进大米爆发病的严重程度.
- 为了确定控制大米爆炸的新策略.
主要方法:
- 酵母二杂交测定以确定AvrPik-D相互作用蛋白质.
- 基因表达分析和酶活性测试.
- 用于亚细胞局部化研究的共聚焦显微镜.
- 产生过度表达OsRBCS4.4的转基因大米系.
主要成果:
- AvrPik-D直接与鲁比斯科小子单元,OsRBCS4.4进行相互作用.
- 过度表达OsRBCS4增强了大米对M. oryzae的抵抗力,并促进了反应性氧物种的产生.
- AvrPik-D抑制了OsRBCS4的转录,并抑制了叶绿体内的Rubisco活动.
- 艾弗皮克-D和OsRBCS4在大米叶绿体中同定位.
结论:
- 玛格纳波特疹效应剂AvrPik-D准并抑制了鲁比斯科小子单元OsRBCS4.4的功能.
- 这种OsRBCS4的抑制破坏了大米的天生的免疫力,促进了M. oryzae感染.
- 准鲁比斯科效应物相互作用是管理爆病的潜在策略.
相关概念视频
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
piRNA - Piwi-interacting RNAs
6.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.8K


