在Populus tremula中的生物化学反应:防御吸和叶昆虫食草动物
Filip Pastierovič1, Alina Kalyniukova1, Jaromír Hradecký1
1Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Kamýcká 129, Suchdol, 165 00 Praha, Czech Republic.
Plants (Basel, Switzerland)
|May 11, 2024
概括
虫显著改变叶的生物化学,减少叶绿素和增加素比昆虫更多. 特定的挥发性化合物区分昆虫攻击的叶子,影响植物生理学.
科学领域:
- 植物生物化学 植物生物化学
- 化学生态化学生态学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 昆虫草食带来的生物压力对植物生理学和生物化学有重大影响.
- 了解植物对不同昆虫养协会 (吸与) 的反应对于植物防御研究至关重要.
研究的目的:
- 为了研究欧洲 (Populus tremula) 叶子对 (Chaitophorus nassonowi) 和海绵 (Lymantria dispar) 感染的生物化学变化.
- 为了比较吸与虫对林,多,叶绿素和挥发性化合物丰富性的影响.
主要方法:
- 对树叶的生物化学分析,包括对林,多化合物,甲和乙的量化.
- 气相色谱-飞行时间质谱 (GC-TOF-MS) 用于挥发性代谢组概况.
- 对照,虫感染和海绵状虫感染的叶子之间的比较分析.
主要成果:
- 在对照组和受昆虫侵袭的叶子之间观察到catechin和procyanidin水平的显著差异.
- GC-TOF-MS分析清楚地区分了对照叶与受虫或海绵虫感染的叶子.
- 区分虫感染的关键挥发性化合物是3-hexenal和5-methyl-2-furanone;对于海绵,它们是trans-α-farnesene和4-cyanocyclohexane.
- 与未受感染的叶子相比,受虫感染的叶子显示出大约50%的较少的叶绿素和两倍的林含量.
结论:
- 虫对树植物生理学的影响比虫更为明显,因为叶绿素和素水平的变化更大.
- 特定的挥发性有机化合物可以作为生物标志物,用于区分虫和海绵虫在欧洲中食草.
- 生物化学分析提供了对植物防御机制的见解,以防止不同类型的昆虫攻击.
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