通过田间生产追踪甜叶曲线病毒:对可持续的甜生产和育种实践的影响
Sharon A Andreason1, Petrina McKenzie-Reynolds1, Kaitlyn M Whitley1
1United States Department of Agriculture, Agricultural Research Service, U.S. Vegetable Laboratory, 2700 Savannah Hwy., Charleston, SC 29414, USA.
Plants (Basel, Switzerland)
|May 11, 2024
概括
早期的甜种植显示低甜叶卷状病毒 (SPLCV) 传播,使得无病毒选择保持. 这为甜的生殖质提供了一种实用的替代方法,而不是繁的病毒消除方法.
科学领域:
- 植物病理学 植物病理学
- 病毒学 病毒学
- 园艺科学 园艺科学
背景情况:
- 甜叶曲线病毒 (SPLCV) 是一种由白传播的贝戈莫病毒,在甜生殖质中引起广泛的感染.
- 目前的病毒消除依赖于耗时的美里系统尖端培养,需要替代方法来有效管理生殖质.
研究的目的:
- 在连续两年内调查甜田种植中的SPLCV传播动态.
- 在选择过程的早期评估识别和保持无病毒甜苗的可行性.
主要方法:
- 甜苗的田间种植是在两年内在季节的早期和晚期进行的.
- 在种植后的各个阶段 (种植后的0,60,120天) 测试了第一年苗木 (FYS) 选择的储存根基的SPLCV.
- 病毒检测包括从精选的阳性样本中测试大量样本和单个植物.
主要成果:
- 在2022年,在早季种植后,只有2.6%的FYS选择对SPLCV呈阳性;在2023年没有检测到阳性FYS.
- 季末种植显示了SPLCV的获取增加,35%的散装样本在60 DAP和52.3%在120 DAP被感染.
- 从积极的散装样本进行单个植物测试,表明感染率低于收获时的散装样本.
结论:
- 在甜种植季的早期,SPLCV的传播压力较低.
- 通过利用早期种植策略,保持无病毒甜的选择是可行的.
- 这种方法为消除病毒和维持生殖质提供了一种可行的替代品,而不是用于消除病毒和维持生殖质.
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