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在Cupriavidus necator B-10646中对和脂肪酸生物合成的多基酸酸盐

Natalia O Zhila^1,2, Kristina Yu Sapozhnikova^1,2, Evgeniy G Kiselev^1,2

¹Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS", 50/50 Akademgorodok, Krasnoyarsk 660036, Russia.

|May 11, 2024

概括

库普里亚维杜斯 (Cupriavidus necator) B-10646有效地从短链和脂肪酸 (SFAs) 中合成多基酸盐 (PHAs). 较短的SFAs (C12-C14) 与较长的链 (C16-C18) 相比,产生显著更高的生物质和PHA生产.

科学领域:

微生物学和生物技术
聚合物科学聚合物科学
代谢工程是代谢工程.

背景情况:

野生型Cupriavidus necator B-10646利用和脂肪酸 (SFAs) 进行生长和聚酸酸盐 (PHA) 合成.
细菌生长和PHA产生受到SFA的碳链长度的影响.

研究的目的:

研究不同和脂肪酸链长度对Cupriavidus necator B-10646生长和PHA合成的影响.
描述由各种SFAs产生的PHAs的特性.

主要方法:

种植Cupriavidus necator B-10646使用劳里克 (12:0),米里斯蒂克 (14:0),棕 (16:0) 和酸 (18:0) 酸作为唯一的碳来源.
生物质和PHA产量的量化.
包括晶度,分子量,化和降解温度在内的PHA属性的表征.

主要成果:

较短的SFA (C12和C14) 与较长的SFA (C16和C18) 相比,支持更高的生物质和PHA产量 (分别高达7.5g/L和75%).
所有测试的SFAs都导致了聚3-基酸 (P(3HB)) 的合成,其结晶度降低 (40-57%) 和典型分子重量 (289-465 kDa).
PHA样本在化点和降解点之间显示出大约100°C的显著温度差距.

结论:

库普里亚维杜斯 (Cupriavidus necator) B-10646证明了从SFAs中有效地产生PHA,较短的链对产量更有利.
这种细菌对SFAs的同化提供了从复杂的含脂肪废物流中合成PHA的潜力.

关键词:

在 PHAs 和 PHAs 之间.生物合成生物合成可降解的多基甲酸.脂肪酸混合物的脂肪酸混合物.脂肪酸脂肪酸脂肪酸脂肪酸属性属性属性属性

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