相关实验视频

Updated: May 7, 2026

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
15:07

VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

Published on: December 28, 2015

26.3K

对于人类·维勒布兰德因子cDNA的分子克隆:通过一种新方法进行身份验证

D C Lynch, T S Zimmerman, C J Collins

    Cell
    |May 1, 1985
    PubMed
    概括

    研究人员确定了一个部分cDNA克隆 (pDL34) 的人类·维勒布兰德因子 (vWf) mRNA. 这种克隆真实地代表了vWf mRNA,提供了一种使用体外转录和翻译来验证cDNA克隆的新方法.

    更多相关视频

    Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation08:30

    Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation

    Published on: August 14, 2017

    10.9K
    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood13:14

    Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

    Published on: February 6, 2018

    10.0K

    相关概念视频

    Southern Blot02:57

    Southern Blot

    Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
    Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
    15.1K
    RACE - Rapid Amplification of cDNA Ends02:35

    RACE - Rapid Amplification of cDNA Ends

    Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
    5.9K