转录的证据表明,Syntrophomonas wolfei和Methanothrix soehngenii之间存在着有利的能量关系
Maaike S Besteman1, Anna Doloman1, Diana Z Sousa1,2
1Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
Environmental microbiology reports
|May 11, 2024
概括
无氧消化过程中的合成相互作用对于甲生产至关重要. 添加像Methanothrix soehngenii这样的酸除尘剂可以改变合成脂肪酸降解剂中的基因表达,从而有可能改善能量代谢.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物化学 生物化学
背景情况:
- 合成性相互作用对于无氧消化和甲生产至关重要.
- 乙基细菌转化脂肪酸,依赖于甲基生物的消耗.
- 在无氧系统中,酸盐交叉养的动态尚不清楚.
研究的目的:
- 为了研究一颗酸盐除尘器对Syntrophomonas wolfei和Methanospirillum hungatei的丁酸盐氧化的影响.
- 分析生长和基因表达的变化,以应对乙酸清理.
主要方法:
- 共同培养Syntrophomonas wolfei与Methanospirillum hungatei,带有或没有Methanothrix soehngenii. 这是一个非常好的方法.
- 监测关键代谢途径的生长和量化基因表达.
- 分析丁酸盐降解和逆电子运输基因表达的变化.
主要成果:
- 酸盐消费率没有受到Metanothrix soehngenii的存在的影响.
- 在Syntrophomonas wolfei中参与丁酸盐降解和反向电子运输的基因在Methanothrix soehngenii存在时表达较低.
- 在Syntrophomonas wolfei中增加IV型-pili操作子的表达表明潜在的直接物种间电子转移.
结论:
- 甲诺德里克斯 (Methanothrix soehngenii) 对Syntrophomonas wolfei的能量代谢产生了积极的影响.
- 包括乙清洁剂在内,对于研究合成脂肪酸降解具有重要意义.
- 在Syntrophomonas wolfei和Methanothrix soehngenii之间可能会发生直接的物种间电子转移.
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