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来自食用的蛋白质复合物作为一种可持续的土豆保护,防止虫害

Karmen Pogačar^1,2, Maja Grundner³, Primož Žigon⁴

¹Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia.

Plant biotechnology journal

|May 11, 2024

概括

菌蛋白 (pleurotolysin A2/B) 通过向昆虫特有的脂质来保护作物. 生产这些蛋白质的转基因马植物有效地阻止了科罗拉多马虫幼虫,提供了一种新的作物保护策略.

科学领域:

农业科学农业科学
生物技术是生物技术.
昆虫学昆虫学是一门学科.

背景情况:

类中的蛋白质复合物, pleurotolysin A2 (PlyA2) 和 pleurotolysin B (PlyB),对科罗拉多马虫 (CPB) 幼虫具有毒性.
这种毒性是通过与昆虫特有的膜脂体的相互作用来调节的.

研究的目的:

开发一种使用植物生产PlyA2/PlyB蛋白质复合物的新型作物保护策略.
创建和评估表达PlyA2和PlyB的转基因土豆植物对CPB耐药性的研究.

主要方法:

构建转基因土豆植物 (cv Désirée),PlyA2 向真空细胞,PlyB 向内质网膜.
对转基因植物和对照植物的CPB幼虫感染,食率,体重和生命周期完成的评估.
对暴露于PlyA2/PlyB复合物的CPB幼虫的基因表达分析.

主要成果:

转基因土豆植物在不影响植物表现的情况下有效地保护免受CPB感染.
食转基因植物的幼虫显著减轻 (5-6倍减少) 的重量,并减少了生命周期完成率 (11%-37%).
幼虫基因表达表明了一般的压力,并且由于脂受体失活,没有耐药性发展的迹象.

结论:

在植物生产的PlyA2/PlyB提供了一个有希望的,可持续的策略来保护作物免受CPB.
该机制针对昆虫特有的脂体,最大限度地降低了耐药性发展的风险.
这种方法表明了利用天然蛋白质复合物用于农业害虫管理的潜力.

关键词:

勒普丁的摄入量下降了.这种植物是Solanum tuberosum.生物杀虫剂生物杀虫剂昆虫的压力反应对于幼虫有毒性的毒性转基因作物是一种转基因作物.

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