在细胞外膀上固脂结的合体结合,用于增强癌症向
Chaoxiang Chen1, Xueping Pan1, Mengdi Sun1
1Department of Biological Engineering, College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian, 361021, China.
Small (Weinheim an der Bergstrasse, Germany)
|May 11, 2024
概括
研究人员开发了一种新的方法,将向性联体连接到牛奶衍生的细胞外囊泡 (mEVs). 这种稳定的表面修饰提高了使用向mEVs的癌症药物递送效率.
科学领域:
- 生物技术是生物技术.
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
背景情况:
- 细胞外囊泡 (EVs) 对药物输送有希望,但缺乏有效的癌症向能力.
- 用细胞结合配体对EV的表面修饰对于有针对性的输送至关重要.
- 现有的联结子结合方法经常遭受不稳定性和功能性改变.
研究的目的:
- 开发一种强大而稳定的方法,用于将连接物与细胞外囊泡 (EVs) 结合.
- 为了提高乳制品EV (mEV) 的癌症向效率,用于药物输送应用.
- 评估结合体修饰的mEVs的稳定性和有效性.
主要方法:
- 使用了Streptomyces phospholipase D和含有maleimide的初级酒精,用于对乳制品EV (mEV) 进行配体固.
- 采用定量纳米流细胞计,以评估连接体修饰的效率和程度.
- 将帕克利塔塞尔装入mEV和合转激素,用于向输送研究.
主要成果:
- 通过数百到数千个连接体,实现了超过90%的mEV的高效表面修饰.
- 在结合,大小和度方面证明了mEV配方的显著长期稳定性.
- 转移素结合的mEV显著增强了细胞吸收和帕克利塔塞尔诱导的细胞毒性.
结论:
- 介绍了EVs的高效,稳定,成本效益和可扩展的连接体结合策略.
- 提供了一个有前途的方法,用于开发下一代使用修改EV的向药物输送系统.
- 开发的方法克服了基于EV的治疗方法的传统连接体结合技术的局限性.
更多相关视频
11:58Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting
Published on: March 8, 2018
7.6K
09:34Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells
Published on: February 9, 2019
9.0K
相关概念视频
Receptor-mediated Endocytosis
104.4K
Overview
104.4K
Transducer Mechanism: Enzyme-Linked Receptors
2.4K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
Major types that are helpful drug targets include:
2.4K
Selectins
3.3K
Cell adhesion is an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.3K
Targeted Cancer Therapies
7.6K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.6K
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K
Lipids as Anchors
5.6K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
5.6K
