板面面向对矿物板表面厚度的影响 通过传输电子显微镜
Henry Schwarcz1,2, Chiara Micheletti3,4, Kathryn Grandfield5,3
1School of Geography and Earth Sciences, McMaster University, Hamilton, ON, L8S 4K1, Canada. schwarcz@mcmaster.ca.
Journal of bone and mineral metabolism
|May 11, 2024
概括
在传输电子显微镜 (TEM) 中倾斜骨段不会显著改变矿物板表面厚度测量. 精确的骨头超结构分析可以在没有切口倾斜的情况下实现,简化了TEM程序.
科学领域:
- 材料科学 材料科学 材料科学
- 生物矿物化 生物矿物化
- 电子显微镜电子显微镜
背景情况:
- 骨超结构分析依赖于传输电子显微镜 (TEM).
- 骨矿物质包括多晶板 (厚度为3-6纳米).
- 矿物板的表面厚度可能随着TEM的截面方向而变化.
研究的目的:
- 调查切口倾斜是否影响骨矿物质板块的表面厚度.
- 为了确定是否需要倾斜来准确的基于TEM的骨矿物质厚度测量.
主要方法:
- 通过离子束磨削制备的皮层人类骨段.
- 在TEM明亮场和HAADF成像中,截面倾斜 (±20°).
- 在各种倾斜角度测量明显的矿物质血小板厚度.
主要成果:
- 表面厚度是±20°倾斜的两倍.
- 最小的表面厚度 (真实厚度) 通过沿板的方向倾斜而发现.
- 矿石板变得越来越少解决超过20°倾斜.
- 类似的平均厚度,从黑色板块图像中获得.
结论:
- 对于精确的骨矿物质板厚度测量,截面倾斜是不必要的.
- 简化的TEM协议可以用于骨超结构分析.
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