鉴定CD5/SOX11双阴性形地幔细胞淋巴瘤
Wen-Yu Chuang1,2,3,4, Hung Chang2,5, Lee-Yung Shih2,5
1Department of Pathology, Chang Gung Memorial Hospital and Chang Gung University, Taoyuan, Taiwan.
概括
CCND1重新排列有助于在循环D1阳性DLBCL病例中识别CD5/SOX11双阴性多形MCL. 高循环D1蛋白和SOX11mRNA表明CCND1的重组,可以通过FISH检测到.
科学领域:
- 血液病理学 血液病理学
- 在瘤学瘤学.
- 分子诊断学 分子诊断
背景情况:
- 循环D1蛋白质阳性扩散型大B细胞淋巴瘤 (DLBCL) 通常缺乏CCND1重组,并且具有DLBCL基因表达特征.
- 罕见的循环D1阳性DLBCL病例表现出CCND1重组以及一些暗示地幔细胞淋巴瘤 (MCL) 的特征.
- 由于缺乏基因表达数据,循环D1阳性DLBCL与CCND1重排和CD5/SOX11双阴性多态MCL之间的区别仍然不清楚.
研究的目的:
- 调查循环蛋白D1蛋白质阳性DLBCL病例的分子和遗传特征,特别是CCND1重组的DLBCL病例.
- 要确定CCND1重排列的案例是否代表一个独立的实体或DLBCL或MCL的子集.
- 建立诊断标准,以识别CD5/SOX11双阴性多形MCL.
主要方法:
- 分析了最初被诊断为循环素D1蛋白阳性DLBCL的8例病例 (有4例和没有CCND1重组的4例).
- 对于环素D1和SOX11的免疫组织化学.
- 全基因组复制号,突变分析和基因表达分析.
- 光现场混合化 (FISH) 用于CCND1重新排列检测.
主要成果:
- 重组CCND1的病例表现出与MCL相似的基因表达特征和拷贝数特征,而非重组的病例类似于DLBCL.
- 所有四个CCND1重组的病例都具有>50%的循环D1蛋白阳性瘤细胞,而只有一个 (25%) 的非重组病例.
- 重组CCND1的病例趋向于更高的SOX11mRNA水平 (P=0.064) 尽管SOX11通过免疫组织化学的消极性.
结论:
- CCND1重新排列是区分CD5/SOX11双阴性多态MCL与循环林D1阳性DLBCL的一个关键特征.
- 对于>50%的循环D1和更高的SOX11mRNA水平的免疫组合化学阳性是CCND1重组的指标.
- 在这些具有挑战性的情况下,FISH是检测CCND1重组的宝贵工具.
关键词:
CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5 CD5环 D1 环 D1 是一种扩散性大B细胞淋巴瘤地膜细胞淋巴瘤是什么繁体形态的 繁体形态的在SOX11中,SOX11是什么?更多相关视频
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