使用一种新型的氨酸脱酶,通过选择性调整形状来制备 (S) -epichlorohydrin
Xiao-Jian Zhang1,2, Meng-Yu Huang1,2, Xin-Xin Peng3
1The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, 18 Chaowang Road, Hangzhou, 310014, People's Republic of China.
工程HheCPS E85P通过减缓反向反应来增强性化水素 (ECH) 合成. 这种策略实现了高产量和99%以上的反体过剩,克服了工业应用中的局限性.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 有机合成 有机合成
- 绿色化学 绿色化学
背景情况:
- 石化化 (ECH) 是制药和化学品的关键中间体.
- 醇脱基酶 (HHDHs) 能够从1,3-二二-2-醇 (1,3-DCP) 中进行异对称的性ECH合成.
- 低光学纯度由于反转环开口限制工业HHDH应用.
研究的目的:
- 开发一种新的策略,利用工程HHDHs来增强性ECH合成.
- 通过调节酶动力学来提高奇拉ECH的光学纯度和产量.
- 了解合环氧化物绿色制造的立体选择性催化机制.
主要方法:
- 使用位点和突变和分子模拟分析来设计HheCPS.
- 开发了一种选择性形状调整策略,以调节前进和反向反应动力学.
- 工程HheCPS E85P突变的特点是其催化效率和立体选择性.
主要成果:
- HheCPS E85P突变体在基质口袋中表现出构造变化,减缓了反向反应.
- 反向反应的催化效率下降了0.23倍,而前进反应效率略有下降.
- HheCPS E85P催化 (S) -ECH合成的产量为55.35%,反体过量从92.54%增加到>99%.
结论:
- 选择性构造调整策略有效地增强了使用工程HHDHs的性ECH生产.
- 工程HheCPS E85P突变克服了以前的HHDH应用中低光学纯度的限制.
- 这项工作为了解立体选择机制和推进性环氧化物绿色制造提供了一条途径.
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