在体外生殖和分化小鼠精子干细胞在脱细胞化的人类胎盘基质
Fatemeh Asgari1,2,3, Hamidreza Asgari1,3, Mohammad Najafi4
1Stem cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran.
概括
人类胎盘组织支架显著增强体外精子生成. 在这些支架上的精子干细胞 (SC) 三维培养促进了细胞增殖和分化标志物.
科学领域:
- 生殖生物学 生殖生物学
- 干细胞生物学 干细胞生物学
- 生物材料科学 生物材料科学
背景情况:
- 进步体外精子生成需要有效的脚手架材料.
- 细胞外矩阵组件提供了有前途的自然支架选项.
- 脱细胞化胎盘组织被探索为生殖应用的潜在生物材料.
研究的目的:
- 为了研究脱细胞化人类胎盘组织作为体外精子生成的支架的有效性.
- 评估2D和3D胎盘支架上的小鼠精子干细胞 (SCs) 的增殖和分化.
- 为了比较3D胎盘支架与2D培养的性能,以支持精子生成.
主要方法:
- 新生小鼠精子干细胞 (SCs) 在2D和3D配置的脱细胞化人类胎盘支架上培养.
- 量化逆转录聚合酶链反应 (qRT-PCR) 用于分析增殖 (Gfrα1) 和分化 (Sycp3,acrosin,Prm1) 标记的基因表达.
- 使用流细胞计量,量化Gfrα1-阳性和Prm1-阳性细胞在不同时间点的百分比.
主要成果:
- 与二维培养相比,三维 (3D) 胎盘支架培养显示出明显更高的Gfrα1基因表达和更高的Gfrα1-阳性细胞百分比.
- 在二维和三维培养中,分化标记物 (Sycp3,Prm1,acrosin) 的表达得到了上调,2D和3D培养中,3D组的表达水平最高.
- 在第35天,Prm1阳性细胞的百分比在3D组 (36.4%) 显著高于2D组 (10.96%).
结论:
- 脱细胞化的人类胎盘组织作为一种可行的和有效的生物材料,用于支持体外精子生成.
- 在胎盘支架上的三维培养显著促进精子干细胞的增殖和分化.
- 胎盘支架代表了一个有前途的生物支架,用于推进体外精子生成领域.
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