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R2R3-MYB转录因子CsMYB60通过调节黄瓜中的黄积累来控制成熟水果的皮肤颜色

Xuewen Xu^1,2, Yu Zhu¹, Ying Yuan¹

¹School of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, Jiangsu, 225009, China.

The Plant journal : for cell and molecular biology

|May 11, 2024

概括

黄瓜果皮肤颜色是由CsMYB60基因控制的. 在CsMYB60中发生的突变,包括外基和内基变化,会影响黄酸的积累,并导致不同的皮肤颜色,这对繁殖有价值.

科学领域:

植物遗传学植物遗传学
果实质量研究果实质量研究
分子生物学分子生物学

背景情况:

黄瓜果皮肤颜色显著影响市场性和消费者吸引力.
多种黄瓜果皮肤颜色 (白色到棕色) 的遗传基础在很大程度上仍未被阐明.
了解水果颜色的遗传基础对于作物改进至关重要.

研究的目的:

为了确定负责控制成熟黄瓜果皮肤颜色的基因.
阐明该基因调节黄类生物合成和皮肤颜色的分子机制.
探索已识别的遗传变异在黄瓜育种中的潜力.

主要方法:

关于天然黄瓜种群的全基因组关联研究 (GWAS).
基于地图的克隆,使用F2种群从黄色棕色和奶油色皮肤父母之间的交叉衍生.
在候选基因中的突变 (外基和内基) 的分析及其对表型的影响.
使用转基因黄瓜植物和分子分析 (RNA-seq,DAP-seq) 的功能验证.

主要成果:

CsMYB60被确定为控制黄瓜果皮肤颜色的关键基因.
在CsMYB60中的外子或内子插入中发生过早停止突变,与分别白色/淡黄色或浅黄色皮肤相关.
过度表达CsMYB60促进了黄类的积累,特别是过氧化物,导致皮肤黄色棕色.
CsMYB60充当转录激活剂,通过促进体结合直接调节像CsF3'H这样的黄类生物合成基因.

结论:

CsMYB60在调节黄瓜水果颜色方面发挥着主导作用.
在CsMYB60中,外来和内在的突变都会导致水果颜色的显著表型变化.
这些发现为未来的黄瓜育种计划提供了有价值的遗传见解,旨在获得理想的水果颜色.

关键词:

在CsMYB60B60中使用.黄瓜黄瓜的使用方法精细地图绘制精细地图绘制黄醇 (Flavonols) 是一种类型的黄.全基因组关联研究研究.皮肤的颜色皮肤的颜色

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