来自茶树油中的terpinen-4-ol可以防止Aspergillus flavus在收获后的小麦谷物中生长
Jing Ren1, Yi-Ming Wang2, Shuai-Bing Zhang1
1School of Biological Engineering, Henan University of Technology, Zhengzhou 450001, People's Republic of China.
International journal of food microbiology
|May 11, 2024
概括
茶树油 (TTO) 和其主要成分,特-4-ol,有效地抑制了阿斯伯吉路斯黄的生长. -4-ol 破坏真菌细胞的完整性,并引起氧化应激,为收获后的保护提供了可持续的解决方案.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 生物化学 生物化学
背景情况:
- 植物挥发性有机化合物 (PVOCs) 是可持续的抗收获后真菌破坏的剂量.
- 茶树油 (TTO) 在防止农产品污染方面表现有前途.
研究的目的:
- 调查TTO及其主要挥发性成分特-4-ol的抗真菌功效,以对抗Aspergillus flavus.
- 阐明特-4-的抗真菌活性背后的机制.
主要方法:
- 使用头空间气体染色学-质谱学提取和分析TTO挥发物.
- 确定TTO和特-4-ol针对A. flavus. 的最小抑制度 (MIC).
- 微观检查真菌形态学,代谢学分析和生物化学测试,以评估细胞损伤和应激反应.
主要成果:
- 在1.714μL/mL时,TTO完全抑制了A. flavus的生长.
- 主要的TTO成分特皮宁-4-ol也完全抑制了A. flavus在0.556μL/mL () 和1.579μL/mL (小麦粒) 的生长.
- 特尔皮宁-4-ol 破坏了菌株形态,血膜完整性,诱导了氧化应激和线粒体功能障碍,改变了A. flavus的关键代谢途径.
结论:
- 特尔皮宁-4-ol是TTO挥发物中的主要抗真菌剂,负责抑制A. flavus.
- 在收获后的应用中,terpinen-4-ol为控制A. flavus提供了一个可行的,自然的替代方案.
- 了解特-4-ol的分子机制为开发新型抗真菌策略提供了洞察力.
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