血激活水通过降低分子量和破坏晶体结构来改善酸酶对酸的可访问性
Yuanchang He1, Chenghui Zhang1, Xueying Zhang1
1College of Food Science and Engineering, Hainan University, Haikou, 570228, China.
Carbohydrate research
|May 11, 2024
概括
血激活水 (PAW) 预处理通过破坏甘油酸结合物来增强基水解. 这种环保方法改善了基的酶分解,产生了更有价值的基托醇糖.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
背景情况:
- 基的有序结构阻碍了其由基酶的水解.
- 与基相比,基氧糖类具有更高的生物活性.
研究的目的:
- 研究血激活水 (PAW) 预处理对素的特性和酶化水解的影响.
- 评估PAW作为一种增强基生物转化方法.
主要方法:
- 使用血激活水 (PAW) 预处理素5分钟.
- 使用Vibrio harveyi (VhChit2) 基因酶对PAW预处理的基进行酶性水解.
- 使用富里埃变换红外光谱法分析物理化学性质,晶体结构和酶性水解.
主要成果:
- 在PAW前处理过程中,糖产量减少了71%;水解度从13%降低到23%.
- VhChit2对基的吸附率从41.7升至58.2毫克/克.
- PAW打破了β-1,4-糖化键,降低了分子量和结晶性,从而增强了酶性水解.
结论:
- PAW预处理是一种简单,有效和环保的基生物转化方法.
- 通过PAW进行增强的基水解会增加高价值基醇糖的产量.
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