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用作饮用水处理过程指标的胡轻斑病毒:目前的形式和表面水中诺罗病毒和轮状病毒的定量关系

Takayuki Miura¹, Syun-Suke Kadoya², Yohei Miura³

¹Department of Environmental Health, National Institute of Public Health, Wako, Japan.

|May 11, 2024

概括

胡轻斑病毒 (PMMoV) 是饮用水源中人类肠道病毒,如诺罗病毒GII和轮状病毒A (RVA) 的可靠指标. 总体而言,PMMoV的度比其他病毒更高,支持其作为指标的使用.

科学领域:

环境微生物学环境微生物学
评估水质水质的评估.
病毒学病毒学

背景情况:

胡轻斑病毒 (PMMoV) 是水中人类肠道病毒的潜在指标.
了解PMMoV的发生及其与诺罗病毒GII (NoV GII) 和轮状病毒A (RVA) 的关系对于饮用水安全至关重要.

研究的目的:

调查PMMoV在日本地表水域的发生,分布和度.
量化评估PMMoV,NoV GII和RVA度之间的关系.
为了确定RVA的基因型多样性,与其度和PMMoV水平相关.

主要方法:

从日本21个饮用水处理厂 (2018-2021) 收集147个地表水样本.
使用实时RT-PCR的PMMoV,NoV GII和RVA溶解和悬浮分量的量化.
使用嵌套PCR和下一代测序进行RVA的基因定型.

主要成果:

在81-100%的样本中检测到PMMoV,其度在3.0~7.0 log10 copies/L.之间.
PMMoV度通常高于NoV GII和RVA,但RVA在18个样本中超过了PMMoV.
动物RVA菌株有助于高遗传多样性,并且与比PMMoV更高的RVA度有关.

结论:

在饮用水源中,PMMoV是NoV GII和人类RVA的合适指标.
评估PMMoV作为一个指标需要与人类和动物感染病毒菌株进行比较.
动物RVA的存在影响PMMoV作为唯一指标的可靠性.

关键词:

饮用水来源饮用水来源大规模的并行测序.这是一个PMMoV.罗塔病毒 (Rotavirus) 是一种病毒.表面的水是表面的水.

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