铁亡:在过早发育的子卵巢中,首次有证据表明由聚乙烯微塑料诱导的铁亡
Waseem Ali1, Yan Chen1, Muhammad Ghiasuddin Shah1
1College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, China.
The Science of the total environment
|May 11, 2024
概括
聚乙烯微塑料 (PVC-MPs) 在子卵巢细胞中诱导铁,导致纤维化和生殖问题. 这项研究强调PVC-MPs对雌性鸟类生殖健康构成威胁.
科学领域:
- 生殖毒理学 生殖毒理学
- 环境健康 环境健康
- 细胞病理学细胞病理学
背景情况:
- 铁亡是一种受调节的细胞死亡形式,与哺乳动物的纤维化和铁代谢障碍有关.
- 铁灭菌在雌性鸟类生殖系统损伤中的作用,特别是由于暴露于微塑料,尚不清楚.
- 聚乙烯微塑料 (PVC-MPs) 是新兴的环境污染物,具有潜在的健康影响.
研究的目的:
- 为了研究由PVC-MPs诱导的铁亡的机制在莫斯科雌性的卵巢组织.
- 为了确定PVC-MP是否会导致卵巢组织纤维化和过早的卵巢衰竭.
- 阐明PVC-MPs对卵巢颗粒细胞功能和类固醇生成的影响.
主要方法:
- 莫斯科雌性被暴露在净化水中,1毫克/升的PVC-MPs或10毫克/升的PVC-MPs两个月.
- 评估了卵巢组织形态,颗粒状细胞活性,原纤维表达,脂质过氧化,甲酸 (MDA) 水平和铁含量.
- 免疫组织化学被用来评估类固醇生成蛋白 (3β-HSD,17β-HSD,CYP11A1,CYP17A1) 和与铁生相关的蛋白质 (ACSL4,COX2,FTH1,SLC7A11,GPX4) 的表达.
主要成果:
- 在卵巢组织中积累的PVC-MPs,损害了颗粒细胞形态和功能,导致毛囊发育障碍.
- 暴露于PVC-MPs下调的类固醇生成蛋白和上调的原纤维,表明卵巢纤维化和功能受损.
- PVC-MPs诱导铁灭菌的特征,包括增加脂质过氧化,MDA水平,铁过载,以及铁灭菌标记物的改变表达 (增加ACSL4,COX2,FTH1;减少SLC7A11,GPX4).
结论:
- 在莫斯科的卵巢颗粒细胞中,PVC-MPs诱导铁亡.
- 这种铁亡导致卵泡发育障碍和卵巢组织纤维化,导致女性生殖功能障碍.
- 调查结果强调PVC-MPs是鸟类雌性生殖健康的重要环境风险因素.
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