相关实验视频

Updated: Jun 26, 2025

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved Non-model Organisms

Published on: May 9, 2017

在新的转录基因组装数据库中,来自七种家用草食动物的100个组织

Yifan Wang^1,2,3, Yiming Huang^1,4, Yongkang Zhen²

¹State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural Reclamation Sciences, Shihezi, 832000, P. R. China.

Scientific data

|May 11, 2024

概括

这项研究对七种食草动物物种的转录组进行了测序,产生了一个有价值的公共数据库. 本资源有助于理解基因表达,以改善畜牧管理和粮食安全.

科学领域:

农业科学农业科学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

化草食动物对于全球粮食安全至关重要,尤其是在边缘土地上.
了解它们的分子生物学可以提高管理和可持续生产.

研究的目的:

为七种食草动物物种生成一个全面的转录组数据库.
为研究草食动物的基因表达和经济特征提供资源.

主要方法:

在100-105个组织上进行了转录基因组测序,这些组织来自七种食草动物物种中的两只雌性.
使用FastQC评估原始和修剪的阅读质量.
存储了大约540亿个高质量的RNA-seq读数进入公共数据库.

主要成果:

在RNA-seq读取中实现了~93.92%的平均映射率.
创建了一个大量的公共转录组数据库,用于多种草食动物物种.
该数据库包含540亿个高质量的配对末端测序读取.

结论:

转录组数据库对于研究草食动物的基因表达和生物通路至关重要.
该资源支持对关键经济特征和可持续畜牧生产的研究.

更多相关视频

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.