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外源葡萄糖干扰抗微生物介导的ROS积累和细菌死亡
Weiwei Zhu1, Lisheng Xiao1, Shouqiang Hong1
1State Key Laboratory of Vaccines for Infectious Diseases, Xiang-An Biomedicine Laboratory & State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Department of Laboratory Medicine, School of Public Health, Xiamen University, 4221-117 South Xiang-An Road, Xiang-An District, Xiamen, Fujian Province 361102, China.
ACS infectious diseases
|May 12, 2024
概括
输液液中的葡萄糖可以降低抗生素对细菌的有效性,这种现象被称为葡萄糖介导的抗菌素耐受性. 这是通过抑制细菌的杀死而不是生长而发生的,并且可以通过特定的代谢产物逆转.
科学领域:
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
- 生物化学 生化学
背景情况:
- 葡萄糖是静脉注射液体配方中常见的成分,通常与抗菌药物一起使用.
- 葡萄糖对抗微生物有效性的影响,特别是杀菌活性,仍然不完全理解.
研究的目的:
- 研究葡萄糖对各种抗菌剂对细菌的活性的影响.
- 阐明通过葡萄糖介导的抗微生物耐受性的潜在机制,并确定潜在的缓解策略.
主要方法:
- 评估了与生理相关的葡萄糖度对不同类抗微生物药物 (昆,氨基甘油酸,素) 存在的细菌存活和生长的影响.
- 研究了活性氧物种 (ROS) 和特定代谢途径 (化果糖代谢,酸途径,TCA循环) 在葡萄糖介导的耐受性中的作用.
- 评估了酸盐和酸在逆转葡萄糖介导耐受性的疗效.
主要成果:
- 葡萄糖显著抑制了针对大肠杆菌的测试抗微生物药物的杀菌活性,而不会影响它们的最小抑制度.
- 葡萄糖降低了普洛素诱导的活性氧物种 (ROS) 激增.
- 缺少pfkA,一种在酸化果糖代谢中的基因,取消了葡萄糖介导的保护,表明其关键作用. 酸盐和酸逆转了这种耐受性.
结论:
- 葡萄糖诱导细菌的耐受性现象,特别是抑制抗微生物杀死而不是生长,称为葡萄糖介导的抗微生物耐受性.
- 这种耐受性与酸化果糖代谢有关,涉及抑制ROS积累.
- 酸盐或酸盐的使用可能是克服在临床环境中通过葡萄糖介导的抗微生物耐受性的新策略,特别是在糖尿病患者中.
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