PiR-hsa-23533通过USP7促进头部和部状细胞癌的恶性瘤
Hanlin Hu1, Jingyu Lu2, Mingjin Xu1
1Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Translational oncology
|May 12, 2024
概括
PIWI交互RNA (piRNA) hsa-23533在头部和部状细胞癌 (HNSCC) 中被上调,通过增强增殖和抑制亡,促进瘤生长. 这种piRNA针对USP7,为HNSCC.提供了潜在的治疗途径.
科学领域:
- 分子瘤学分子瘤学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 头部和部状细胞癌 (HNSCC) 的进展涉及遗传和表观遗传变化.
- PIWI相互作用RNAs (piRNAs) 在瘤发生中发挥作用,但它们在HNSCC中的特定功能尚未完全理解.
研究的目的:
- 研究piRNAs在HNSCC中的作用和分子机制.
- 为了确定参与HNSCC发育和进展的特定piRNAs.
主要方法:
- RNA测序以识别差异表达的piRNAs.
- 定量实时PCR和RNA在现场杂交以评估piR-hsa-23533的表达.
- 在体外和体内测试以评估piR-hsa-23533对HNSCC细胞增殖和细胞亡的功能影响.
- RNA下拉和RNA免疫沉降测试以确定分子相互作用.
主要成果:
- 发现piR-hsa-23533在HNSCC细胞和组织中被上调.
- 过度表达piR-hsa-23533促进了HNSCC细胞的增殖和抑制了HNSCC细胞的亡,无论是体外还是体内.
- 沉默piR-hsa-23533显示出相反的效果.
- 在机械上,piR-hsa-23533与乌比基特异性蛋白酶7 (USP7) 结合,增加其mRNA和蛋白质表达.
结论:
- piR-hsa-23533在HNSCC中发挥着重要的功能作用.
- 这些发现表明piR-hsa-23533是抗HNSCC策略的潜在治疗点.
更多相关视频
07:29Intramucosal Inoculation of Squamous Cell Carcinoma Cells in Mice for Tumor Immune Profiling and Treatment Response Assessment
Published on: April 22, 2019
11.5K
07:52In Vivo CRISPR/Cas9 Screening to Simultaneously Evaluate Gene Function in Mouse Skin and Oral Cavity
Published on: November 2, 2020
6.4K
相关概念视频
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:03Rous Sarcoma Virus (RSV) and Cancer
5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.1K
01:06Induced Pluripotent Stem Cells
4.0K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.0K
02:33Hedgehog Signaling Pathway
7.3K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.3K