聚糖利用位点编码为DUF1735,可能作为碳水化合物活性酶的膜结合间隔器
Lisanne Hameleers1, Lucie A Gaenssle1, Salvador Bertran-Llorens2
1Department of Bioproduct Engineering, University of Groningen, The Netherlands.
FEBS open bio
|May 12, 2024
概括
不知功能的域1735 (DUF1735) 蛋白质在多糖利用位点 (PULs) 中被发现,但它们的功能尚不清楚. 我们的分析显示,DUF1735可能作为PUL中的膜结合N终端域,用于碳水化合物利用.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 蛋白质生物信息学 蛋白质生物信息学
背景情况:
- 具有未知功能的域1735 (DUF1735) 的蛋白质在多糖利用位点 (PUL) 中很常见.
- 在这些位点内,DUF1735蛋白的特定作用尚不清楚.
研究的目的:
- 使用计算分析功能预测DUF1735.
- 研究DUF1735.5的基因组背景和蛋白质结构.
主要方法:
- 开发域名和附近分析器程序.
- 基因和基因组的京都百科全书 (KEGG) 和UniProt数据库的采矿.
- 对DUF1735分布和与PULs相关的基因组分析.
主要成果:
- DUF1735仅在 Bacteroidota 基因组中发现,其中 Bacteroides thetaiotaomicron 含有 46 个副本.
- 97.8%的DUF1735蛋白质被编码在PUL中,通常是多模块蛋白的N-终端.
- DUF1735与已知的域具有结构相似性,并具有预测的脂蛋白信号.
结论:
- DUF1735可能作为一个膜结合的N端间隔器域.
- 这个域促进了参与PUL介导的碳水化合物代谢的多种C端域的相互作用.
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