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米尔斯坦和科勒:单克隆抗体

J V Pai-Dhungat1

  • 1Professor of Medicine (Retd), Department of Medicine, Topiwala National Medical College and Bai Yamunabai Laxman Nair Charitable Hospital; Honorable Physician, Bhatia Hospital, Mumbai, Maharashtra, India.

The Journal of the Association of Physicians of India
|May 13, 2024
PubMed
概括

诺贝尔奖获得者塞萨尔·米尔斯坦 (César Milstein) 是抗体研究的先驱. 他在医学研究委员会 (MRC) 的工作显著推进了分子生物学和免疫学.

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...