在玉米和大米中发现共存的病毒衍生的siRNA,这些大米被黑条纹矮人病毒感染
Feifei Wang1, Zhennan Xu1, Ronggai Li2
1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plant disease
|May 13, 2024
概括
米黑条纹矮人病毒在玉米和米中产生共享的小干扰RNA (siRNA),影响宿主基因表达. 这一发现为开发针对植物疾病的抗病毒策略提供了新的途径.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 米黑条纹矮人病毒 (RBSDV) 导致玉米和米的大量作物损失.
- 病毒衍生小干扰RNAs (vsiRNAs) 在植物病毒共同进化的军备竞赛中至关重要,通过RNA沉默调节宿主免疫力.
- RBSDV在不同宿主物种中产生保存的 vsiRNA的能力仍未得到研究.
研究的目的:
- 调查RBSDV是否会在不同植物物种,特别是玉米 (Zea mays) 和大米 (Oryza sativa) 中产生常见的 vsiRNA.
- 描述这些宿主中由RBSDV产生的 vsiRNAs,并确定它们的潜在目标.
- 探索这些发现对开发新型抗病毒策略的影响.
主要方法:
- 来自受感染的玉米和大米的小RNA和退化体库的高通量测序.
- 对病毒基因组上的 vsiRNA特征,分布和热点的生物信息分析.
- 干环PCR和实时定量PCR用于验证特定共存的siRNAs (co-vsiRNAs) 的功能.
主要成果:
- 在玉米和大米中,RBSDV感染主要产生21和22nt的 vsiRNA.
- 在两种宿主中确定了73种共同vsiRNA,其中特定的共同vsiRNA (3.1和3.5) 已被证实抑制宿主基因表达.
- 病毒感染对与玉米中的核糖体功能和大米中的代谢/生物合成相关的基因产生了差异性影响.
结论:
- RBSDV可以产生在多种宿主物种中起作用的保存的 vsiRNA.
- 这些保存的 vsiRNAs 在抑制宿主基因表达方面发挥作用,有助于病毒病原性.
- 这些发现为开发跨物种抗病毒疗法提供了基础,该疗法针对保存的 vsiRNA.
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