通过气体染色体-离子移动性光谱学优化通过尿液挥发性物质的非定向分析协议. 一个试点研究研究
S Novakova1, B Czippelova2, E Baranovicova2
1Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovak Republic. slavomira.novakova@uniba.sk.
概括
尿液中的挥发性有机化合物 (VOC) 是非侵入性诊断的关键. 这项研究发现,尿液成分在不同的温度和解条件下保持稳定,确保可靠的VOC分析用于代谢分析.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 临床诊断 临床诊断 临床诊断
背景情况:
- 生物样本中的挥发性有机化合物 (VOC) 为非侵入性疾病诊断和代谢分析提供了潜力.
- 尿液是适合临床分析的生物液体,因为它很容易获得.
- 提高从有限的尿液头部空间提取VOC并确保样品处理期间的稳定性至关重要.
研究的目的:
- 为了研究化时间,温度和解对尿液挥发性的影响.
- 为了监测在不同分析前条件下选择的VOC的稳定性.
- 为了优化尿样准备可靠的VOC分析.
主要方法:
- 使用BreathSpec®装置分析挥发性有机物,集成气体染色学 (GC) 和离子运动谱学 (IMS).
- 对不同化时间和温度进行系统测试.
- 对尿样品的三种不同的解条件的评估.
主要成果:
- 在34种挥发性有机化合物中,只有9种在测试温度和化时间之间观察到具有统计学意义的差异.
- 在任何三个解条件下都没有检测到VOC的统计学意义上的变化.
- 尿液的VOC档案在受到热应力和解时表现出相对稳定性.
结论:
- 尿液的组成,特别是其挥发性,在各种热应力和解过程中基本稳定.
- 这些发现支持尿液VOC分析用于诊断和代谢分析应用的可靠性.
- 分析前的处理条件,特别是解,似乎对尿液的VOC稳定性影响很小.
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