一个血管化的活体黑色素瘤模型,适合用于不同瘤阶段的转移研究,使用从根本上不同的生物墨水
Rafael Schmid1, Sonja K Schmidt2, Stefan Schrüfer3
1Laboratory for Tissue-Engineering and Regenerative Medicine, Department of Plastic and Hand Surgery, University Hospital of Erlangen, Krankenhausstraße 12, 91054, Erlangen, Germany.
Materials today. Bio
|May 13, 2024
概括
3D生物打印模型提供了改进的瘤分期和转移研究. 不同的生物墨水创造出不同的瘤微环境,使得研究黑色素瘤的进展,休眠和治疗反应 in vivo.
科学领域:
- 生物医学工程 生物医学工程
- 癌症研究 癌症研究
- 在3D生物打印中使用3D生物打印
背景情况:
- 传统的二维癌症模型对药物开发缺乏体内相关性.
- 3D生物打印为研究复杂的瘤行为提供了增强的模型.
- 黑色素瘤转移研究需要复杂的模型和受控的微环境.
研究的目的:
- 在一个血管化的3D模型中分析生物墨水模仿不同的黑色素瘤瘤阶段.
- 为了研究生物油墨特性对瘤进展,休眠和转移的影响.
- 为体内转移研究建立一个封闭的,血管化的系统.
主要方法:
- 在体内利用造血管化的动脉静脉循环黑色素瘤模型.
- 评估了生物油墨成分,可打印性,刚性和孔隙结构.
- 采用光片光显微镜和组织学分析 (HMB-45,CD163,Ki67,MITF/BRN2,ATX,MMP3) 的方法.
主要成果:
- 生物墨水显著影响瘤阶段:Matrigel/Alg/HA/Gel用于进展,Cellink生物墨水用于休眠状态.
- 马特里格尔显示主要瘤的生长明显高于Cellink Bioink.
- 组织学证实了黑色素瘤阶段,进展油墨显示高增殖,巨细胞透和转移;即使在Cellink Bioink中初级瘤生长最小,也观察到淋巴结转移.
结论:
- 3D生物打印模型有效地复制了各种黑色素瘤瘤阶段和微环境.
- 这种模型适用于研究瘤病理学,转移和评估体内治疗.
- 生物墨水选择对于控制瘤行为和实现特定研究目标至关重要.
相关概念视频
Pulmonary Tuberculosis I
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Pulmonary Tuberculosis II
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Pulmonary Tuberculosis III
Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
The first classification is based on the development of the disease, and it includes the following categories:
Pulmonary Tuberculosis IV
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Pulmonary Tuberculosis V
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...


