Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Microarrays02:34

DNA Microarrays

17.3K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
17.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Genetic diversity within Miscanthus × giganteus: Evidence from morphological traits and ITS rDNA in European accessions.

Molecular biology reports·2026
Same author

Copper is an intestinal habitat filter affecting the gut microbiota interactions with Salmonella Typhimurium.

Microbiome·2026
Same author

HaDeX2: multi-dimensional analysis of hydrogen-deuterium exchange mass spectrometry data.

Bioinformatics (Oxford, England)·2026
Same author

Comparison of manual with artificial intelligence-aided interpretation of ANA HEp-2 IIF assay patterns in a clinical diagnostics lab.

Clinica chimica acta; international journal of clinical chemistry·2026
Same author

Cannabidiol does not cause DNA double-strand breaks in a human liver-derived cell model.

Journal of cannabis research·2025
Same author

MicroRNAs in neurodegenerative diseases: from molecular mechanisms to clinical biomarkers, detection methods and therapeutic strategies-advances and challenges.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2025

相关实验视频

Updated: Jun 26, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K

在处理NanoString nCounter数据的挑战和机遇.

Jarosław Chilimoniuk1, Anna Erol1, Stefan Rödiger2

  • 1Clinical Research Centre, Medical University of Białystok, Białystok, Poland.

Computational and structural biotechnology journal
|May 13, 2024
PubMed
概括

用于mRNA和miRNA研究的NanoString nCounter平台因缺乏标准化分析而面临着停滞不前的受欢迎程度. 本研究评估了R包,以建立nCounter数据的最佳数据处理工作流.

关键词:
背景纠正 背景纠正 背景纠正不同表达式的差异表达式在NanoString中使用.规范化 规范化 规范化它们是mRNARNA.这是一个小RNARNA.n 计数器 计数器nSolverver 是一个解决方案.

更多相关视频

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

8.3K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.8K

相关实验视频

Last Updated: Jun 26, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

13.7K
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
10:37

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

8.3K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

11.8K

科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 基因组学就是基因组学.

背景情况:

  • 该NanoString nCounter是一个中等通量平台,用于mRNA和miRNA差异表达分析.
  • 它提供了诸如没有放大步骤和低质量样本分析等优势.
  • 平台的受欢迎程度已经稳定,可能是由于缺乏标准化的分析管道.

研究的目的:

  • 为了标准化对nCounter数据分析工作流程的描述.
  • 为了评估用于nCounter数据处理的R包.
  • 为mRNA和miRNA样本分析提供最佳方法的指导.

主要方法:

  • 将nCounter数据分析分为五个步骤:预处理,质量控制,背景校正,规范化和微分表达式分析.
  • 在这些步骤中对11个R包的功能进行了评估.
  • 评估了mRNA和miRNA研究的包装申请.

主要成果:

  • 确定了11个R包用于nCounter数据分析的功能.
  • 提供了关于对mRNA和miRNA样本应用这些R包的评论.
  • 强调需要标准化工作流程.

结论:

  • 标准化数据分析工作流对于NanoString nCounter平台至关重要.
  • 对R包的评估为优化nCounter数据处理提供了指导.
  • 这项工作旨在支持研究人员最大限度地发挥nCounter技术的实用性.