在Streptomyces mobaraensis中通过系统的工程策略有效地生物合成转胺酶
Fang Yuan1, Guoying Li2, Zilong Li3
1The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
Current research in food science
|May 13, 2024
概括
研究人员在Streptomyces mobaraensis中工程转质氨酶 (TGases),实现了创纪录的高酶活性. 这一突破提高了工业酶的生产,并扩大了TGase在食品行业的应用.
科学领域:
- 合成生物学 合成生物学
- 酶工程是什么? 酶工程是什么?
- 工业生物技术 工业生物技术
背景情况:
- 转胺酶 (TGases) 是关键的工业酶,可催化蛋白质交联.
- 增强TGase生物合成对于扩大其在食品和药品中的应用至关重要.
- 像Streptomyces这样的微生物宿主中的重组表达提供了一个可扩展的生产平台.
研究的目的:
- 开发一个高效的系统工程策略,用于增强TGas合成.
- 为了优化TGase TG_LD在重组性Streptomyces mobaraensis菌株中的异质表达.
- 为了实现工业应用的高水平TGas生产.
主要方法:
- 对TGase TG_L2020和TG_LD的酶特性进行比较分析.
- 系统基因工程包括促进物优化,信号基因修饰和表达盒放大.
- 大规模发酵 (1000升) 的工程化Streptomyces mobaraensis smL2020ΔTG菌株.
主要成果:
- 工程TG_LD在摇瓶中达到56.43U/mL,在1000L发酵器中达到63.18U/mL的前所未有的活性.
- 这种重组菌株在20个世代内表现出高基因稳定性.
- 优化的表达策略显著促进了TGase生物合成.
结论:
- 系统工程策略对于改善工业酶生物合成是非常有效的.
- 开发的方法为可扩展的TGas生产提供了强大的解决方案.
- 增强的TGas生产将促进食品工业及其他领域的更广泛应用.
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