在SARS-CoV-2基因特异性CT值和COVID-19相关的医院死亡率之间存在关联
Mpho L Sikhosana1,2, Richard Welch3, Alfred Musekiwa4
1Department of Virology, National Health Laboratory Service, Charlotte Maxeke Johannesburg Academic Hospital, Johannesburg, South Africa.
Frontiers in epidemiology
|May 13, 2024
概括
较低的SARS-CoV-2聚合酶连锁反应 (PCR) 周期值 (Ct) 值表明病毒载量较高,并与COVID-19死亡风险增加有关. 然而,Ct值因测试平台而异,限制了直接比较.
科学领域:
- 病毒学 病毒学
- 传染性疾病 传染性疾病
- 公共卫生 公共卫生
背景情况:
- 没有特定的SARS-CoV-2生物标志物用于预测疾病严重程度.
- 对使用SARS-CoV-2 PCR循环值 (Ct) 来预测疾病进展的兴趣很高.
研究的目的:
- 评估COVID-19病例的住院死亡率与SARS-CoV-2特定基因标Ct值之间的关联.
- 分析不同流行病波的死亡率趋势及其与Ct值的相关性.
主要方法:
- 来自高省的住院COVID-19病例的回顾性分析 (2020年4月至2022年7月).
- 在不同测试平台 (Roche,GeneXpert,Abbott) 中对Ct值 (低:<20,中:20-30,高:>30) 的分类.
- 统计分析调整性别,年龄和并发症以确定死亡率的几率.
主要成果:
- 总共分析了1205例病例,记录了296例 (24.6%) 的死亡,主要是在第1波中.
- 与较低的Ct值观察到更高的死亡率:Ct20-30 (aOR2.25) 和Ct<20 (aOR3.18) 与Ct>30.0相比.
- 平均Ct值在不同平台上有很大的差异:罗氏26,GeneXpert38和Abbott21.
结论:
- 较低的SARS-CoV-2 Ct值 (<30) 与增加COVID-19相关死亡的几率有关.
- 不同测试平台的CT值无法直接比较,这限制了它们在预测疾病严重程度方面的普遍应用.
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